NEFA和BHBA对体外培养的脂肪细胞HSL、ADPN mRNA表达的影响  

Effects of NEFA and BHBA on expression of HSL mRNA and ADPN mRNA in vitro culture adipocyte

作　　者：张辉[1] 丛立新[2] 张才[1] 王哲[1] 

机构地区：[1]吉林大学畜牧兽医学院,吉林长春130062 [2]吉林农业科技学院动物科学系,吉林吉林市132101

出　　处：《中国兽医学报》2008年第7期840-843,共4页Chinese Journal of Veterinary Science

基　　金：国家自然科学基金资助项目(30571365)

摘　　要：在体外培养的牛脂肪组织和脂肪细胞中,分别添加5个浓度梯度的NEFA和BHBA,均设3个重复,通过荧光定量PCR技术,观测不同浓度的NEFA和BHBA对牛脂肪细胞ADPN mRNA与HSL mRNA丰度的影响。结果表明:NEFA在一定浓度范围内(0.2~0.8 nmol/L)对ADPN mRNA表达有显著促进作用并呈剂量依赖性,而高浓度(>1.6 nmol/L)时又显著下调其表达;0.2~0.8 nmol/L NEFA对HSL mRNA的表达有抑制作用,呈剂量依赖性,但在高浓度时(>0.8 nmol/L)反而促进了其表达(P<0.05)。低浓度的BHBA对HSL mRNA的表达无显著抑制作用,高浓度(1.2 mmol/L)的BHBA显著下调HSL mRNA的表达,并呈剂量依赖性;低浓度(<0.6mmol/L)的BHBA对ADPN mRNA的表达无明显作用,但高浓度的BHBA(>0.6 mmol/L)对ADPN mRNA的表达具有明显的抑制作用(P<0.05)。结论:代谢中间产物可通过促进ADPN mRNA或抑制HSL mRNA的表达来调节脂肪代谢。To investigate whether NEFA and BHBA affect the expression of ADPN mRNA and HSL mRNA in adipose cell from a newborn calf,five different levels of NEFA and BHBA were added to the media.Abundance of ADPN mRNA and HSL mRNA in adipose cells was determined by real-time quantitative RT-PCR.The results showed that NEFA up-regulated the level of ADPN mRNA significantly,and this effect have a dose dependent in the concentration 0.2 nmol/L to 0.8 nmol/L,and down-regulated when the concentration of NEFA exceed 1.6 nmo...

关 键 词：非酯化脂肪酸 β-羟基丁酸 牛脂肪细胞 HSLmRNA ADPN MRNA 

分 类 号：S858.23[农业科学—临床兽医学]