中等毒力新城疫病毒ND03-026株全基因组遗传特性分析  

Genome and genetic characterization analyses of mesogenic isolate of Newcastle disease virus ND03-026 strain

作　　者：刘华雷[1] 包静月[1] 郑东霞[1] 孙旭辉[2] 吴延功[1] 王志亮[1] 

机构地区：[1]中国动物卫生与流行病学中心,山东青岛266032 [2]南京农业大学动物医学院,江苏南京210095

出　　处：《中国兽医学报》2008年第7期743-747,共5页Chinese Journal of Veterinary Science

基　　金：农业部国家新城疫参考实验室职能性资助项目

摘　　要：从2003年临床发病鸡群当中分离到1株NDV,经致病指数测定为中等毒力。通过RT-PCR技术扩增了其F基因主要功能区片段,并构建了遗传进化树,发现其在分类地位上属于基因Ⅱ型,与我国现用的疫苗株LaSota处于不同的亚群,且裂解位点的组成为典型强毒株序列。对其全基因组序列进行测定,结果已登陆GenBank,登录号为DQ060053。此分离株基因组由15 186个核苷酸组成,编码6种结构蛋白,顺序为3-′NP-P-M-F-HN-L-5′。通过对其6个编码区进行遗传进化与核苷酸和氨基酸同源性分析,结合致病性结果发现其与Roakin毒株的遗传关系最近。One strain of Newcastle disease virus(NDV) isolated from outbreak in chicken in 2003 was characterized pathotypically and genotypically.The strain was mesogenic according to the pathogenetic tests.The F protein plays an important role in determining the virulence of NDV strains.The main functional region of the F gene was amplified by RT-PCR and sequenced.For genotyping,phylogenetic tree based on the nucleotide 47-420 of the F gene were performed using sequences of our isolate and sequences of selected NDV ...

关 键 词：新城疫病毒 中等毒力 基因组测序 遗传进化 同源性分析 

分 类 号：S852.65[农业科学—基础兽医学]