表没食子酸儿茶素通过抑制MAP kinase及AP-1/NF-KB阻止RAW264.7细胞受钛颗粒刺激而释放TNF-a的效果  

作　　者：金山 金京春 

机构地区：延边第二人民医院,133000

出　　处：《中外健康文摘：临床医师》2008年第6期1-4,共4页

摘　　要：目的:探讨人工假体分离出的颗粒碎片刺激巨噬细胞而释放的肿瘤坏死因子(TNF-α)在人工髋关节术后引起骨溶解作用机理及EGCG效果。方法:采用钛颗粒刺激巨噬细胞而释放TNF-α模型及酶联免疫吸附剂测定、蛋白质印迹、凝胶迁移率实验来研究其生物分子学机制,以及EGCG对此机制的影响。结果:鼠类巨噬细胞受钛颗粒刺激而释放TNF-α及绿茶里的多苯成份EGCG(epigallocatechin gallate)表没食子酸儿茶素对TNF-α合成具有抑制作用,并此阻止由抑制JNK活性及IkB降解有关。而且,JNK抑制剂SP600125和NF-kB抑制剂MG132大大抑制了钛颗粒诱导的TNF-α释放,而p38抑制剂无此作用,说明TNF-α合成与JNK及NF-kB通路有关,甚至,EGCG阻止钛颗粒诱导而激活的AP-1和NF-Kb。这种结果证明了钛颗粒诱导的TNF-α释放JNK/AP-1及NF-kB通路有关,而且EGCG对钛颗粒诱刺激而释放的TNF-α的抑制是通过阻止JNK/AP-1和NF-Kb通路有关。结论:EGCG可能对人工髋关节置换术后产生的骨溶解及假体松动具有预防及治疗作用。Objective:To study the mechanism of osteolysis after total hip replacement by tumor necrosis factor-α(TNF-α)release in macrophages stimulated with particulate debris from prosthesis and the effect of EGCG with TNF-α.Method:A model of TNF-α release by stimulating macrophages with titanium particles was made and study the mechanism of TNF-α and effect with TNF-α by Elisa/West blot/EMSA.Result:TNF-α was released in murine macrophage stimulaged by titanium(Ti)particles and(-)-epigallocatechin gallate(EGCG),a ma...

关 键 词：肿瘤坏死因子 表没食子酸儿茶素 钛颗粒 人工髋关节术 骨溶解 

分 类 号：R285.5[医药卫生—中药学;医药卫生—中医学]