应用TaqMan-MGB探针进行松材线虫的实时荧光定量检测技术研究  

Quantity assay of Bursaphelenchus xylophilus with TaqMan-MGB probe

作　　者：葛建军[1] 曹爱新[1] 刘先宝[2] 陈长法[3] 

机构地区：[1]中国检验检疫科学研究院,北京100029 [2]华南热带农业大学,海南571737 [3]山东出入境检验检疫局,青岛266002

出　　处：《植物病理学报》2005年第S1期52-58,共7页Acta Phytopathologica Sinica

摘　　要：松材线虫是国际公认的最重要的检疫性有害生物之一,也是我国2类检疫危险性有害生物,我国口岸多次从货物的木质包装中截获该线虫。由于松材线虫与拟松材线虫在形态上极其相似,难以区分,幼虫更无法用于鉴定。传统的形态学鉴定、生化以及其他分子技术等方法存在费时、准确度不高、灵敏度低等缺点,不易形成标准。我们设计筛选一对引物以及一条MGB探针,对松材线虫进行实时荧光PCR检测。建立了一条从1 pg到104pg标准曲线,相关系数r=0．965。该检测方法省时、准确、快速、无污染。Pine wood nematode (Bursaphelenchus xylophilus), one of the most important quarantine pests in the world, is also in the A2 list concerned to China. Entry port of China intercepted it for hundreds times from wood packaging materials of goods. Due to its morphological similarity with Bursaphelenchus mucronatus, it is difficulty to distinguish it from B. mucronatus. Many attempts to develop detecting and diagnosing methods for Bursaphelenchus xylophilus had been conducted, including biochemical technology and...

关 键 词：定量检测 松材线虫 MGB 核糖体ITS 

分 类 号：S41-35[农业科学—植物保护]