大豆猝死综合症病菌检测技术研究  

The detection techniques for sudden death syndrome pathogens of soybean

作　　者：吴品珊[1] 严进[1] J.C.Rupe S.Giammaria E.J.Boza 

机构地区：[1]中国检验检疫科学研究院动植物检疫研究所 [2]University of Arkansas Fayetteville AR 72701 USA

出　　处：《植物病理学报》2005年第S1期28-34,共7页Acta Phytopathologica Sinica

摘　　要：大豆猝死综合症是美国等几个大豆主产国的一种较新的、危害性很大的大豆真菌病害,它分别由两种镰刀菌Fusari- um virguliforme和Fusanum tucumaniae引致。本研究确立了从土壤中筛选、分离大豆猝死综合症病菌的选择性培养基 PCNB;明确了中国具有对该病菌敏感的大豆品种;利用Southern杂交技术,将致病菌和近似菌的基因组DNA分别用EcoRI 酶切,与mtDNA探 2u18和4u40杂交的RFLP图谱可成功的鉴定F. virguliforme,F.tucumaniae和近似菌F.phaseoli。由此建立了,通过选择性培养基分离进口大豆中的土壤,进而以Southern杂交技术鉴定大豆猝死综合症病菌的检测方法。Soybean Sudden Death Syndrome caused by Fusarium virguliforme and Fusarium tucumaniae respectively is a relatively new and severe soybean disease distributed in United States and some other main soybean produce countries. A modified selective culture medium named PCNB was obtained to isolate and enumerate the pathogens from soil . Some Chinese soybean varieties were proved sensitive to the pathogens. Total DNA of the 2 pathogens and their similar ones digested with EcoR I, were hybridized with mtDNA probes ...

关 键 词：大豆猝死综合症 检测 

分 类 号：S435.651[农业科学—农业昆虫与害虫防治]