云南泡桐丛枝病植原体核糖体蛋白基因片段序列分析  

Sequencing of ribosomal protein gene of phytoplasma associated with Paulownia witches'-broom in Yunnan province

作　　者：和志娇[1] 蔡红[1] 陈海如[1] 和根强[2] 吴华英[1] 唐丽明[1] 

机构地区：[1]云南农业大学 云南省植物病理重点实验室,昆明650201 [2]云南省潞西市市农业局经济作物技术推广站,芒市678400

出　　处：《植物病理学报》2005年第S1期18-21,共4页Acta Phytopathologica Sinica

基　　金：国家自然科学基金资助项目(30260064)云南省自然科学基金资助项目(2000C0014Q)

摘　　要：应用植原体核糖体蛋白基因通用引物对rpF1/rpR1,对采自云南省曲靖市的泡桐丛枝病植原体DNA(PaWB-QJ)进行PCR扩增,得到1．3 kb的特异片段,证明此病株中存在植原体。将此片段与pGEM-T Easy载体连接并转化大肠杆菌 JM109感受态细胞,进行PCR鉴定、核糖体蛋白基因部分核苷酸序列测定及分析。结果表明,该株系(PaWB-QJ)核糖体蛋白基因片段长1 244 bp,包含rps19、rp122和rps3基因。对PaWB-QJ株系的核糖体蛋白基因序列的同源性比较结果显示与 16S rI-B亚组的翠菊黄化(Aster yellows,AY)、长春花黄化(Periwinkle yellows,PY)和泡桐丛枝德国株系(Paulownia witches’-broom,PaWB-German)的亲缘关系最近,达到99．0％以上,而与其它组中的株系明显低于97．0％,所以认为该植原体株系属于翠菊黄化组B亚组(16SrI-B)。Universal primer (rpFl/rpRl) for ribosomal protein gene was used to detect phytoplasma of paulownia witches -broom(PaWB-QJ) by polymerase chain reaction and DNA fragment of 1. 3 kb was amplified. The result indicated existence of phytoplasma associated with the diseased plant. The amplified fragment was liga-ted into the competent cell of E. coli JM109 strain. Cloned DNA fragments were verified by PCR and nucleo-tide sequence analysis. The result indicated that the ribosomal protein gene of PaWB-QJ strain c...

关 键 词：泡桐 丛枝 核糖体蛋白基因序列 植原体 翠菊黄化 

分 类 号：S763.7[农业科学—森林保护学]