葡萄的两种检疫性病毒的多重RT-PCR检测  

Detection the two quarantine viruses infecting grapevine by multiple RT-PCR

作　　者：薛杨[1,2] 安德荣[1] 李明福[2] 

机构地区：[1]西北农林科技大学植保学院 [2]中国检验检疫科学研究院动植物检疫研究所,北京100029

出　　处：《植物病理学报》2005年第S1期14-17,共4页Acta Phytopathologica Sinica

摘　　要：本研究利用多重RT-PCR技术,对葡萄的检疫性病毒中番茄环斑病毒(ToRSV)和烟草环斑病毒(TRSV)进行检测, 并研究了反应体系中各个参数对多重RT-PCR的影响。结果表明:可以在同一反应体系中实现对ToRSV和TRSV的RT- PCR检测,但模板浓度、引物浓度、循环数等3个因素对检测结果的准确性有较大的影响,3个参数的增加都会导致非特异性扩增的增多,而dNTP以及Taq酶的浓度对实验结果并无较大影响。Multiple RT-PCR was applied to detect the import quarantine viruses on grapevine: Tomato ring-spot virus (ToRSV), Tobacco ringspot virus (TRSV). The factors affecting RT-PCR reaction of this system were studied,too. The results indicated ToRSV and TRSV could be detected by this method successfully, and three ingredients; template concentration, primer concentration and cycles had more influences on the results in the system than that of dNTP concentration and Taq DNA polymerase concentration.

关 键 词：多重RT-PCR 番茄环斑病毒(ToRSV) 烟草环斑病毒(TRSV) 

分 类 号：S436.631.12[农业科学—农业昆虫与害虫防治]