狼疮方对慢性GVHD狼疮样肾炎小鼠模型的作用  

Observation of Lupus Recipe in Treating Lupus Nephritis Mice with Chronic Graft Versus Host Disease

作　　者：阳晓[1] 魏毅[2] 叶任高[1] 梁鸣[1] 陈伟英[1] 李幼姬[1] 孔庆瑜[1] 余学清[1] 

机构地区：[1]中山大学附属第一医院肾内科,广州510080 [2]第一军医大学

出　　处：《中国中西医结合杂志》2005年第S1期74-77,共4页Chinese Journal of Integrated Traditional and Western Medicine

基　　金：国家自然科学基金(No.39970933;No.30271668);高等学校博士学科点专项科研基金(No.2002055805);广东省科委重点攻关项目(No.2003C30202);广东省自然科学基金(No.990073)

摘　　要：目的探讨狼疮方治疗狼疮肾炎的有效性及作用机理。方法采用慢性移植物抗宿主病(GVHD)狼疮样小鼠模型,经狼疮方、强的松治疗后分别检测血清抗ds-DNA抗体、脾细胞CD40表达及肾脏功能,并观察形态学变化。结果(1)血清抗dsDNA抗体为模型组与正常对照组比较差异有显著性(P＜0．01)；狼疮方组及强的松组与正常对照组比较差异无显著性(P＞0．05)。(2)脾细胞CD40表达的平均荧光强度及阳性细胞百分率模型组均显著高于正常对照组(P＜0．05或P＜0．01)；狼疮方组及强的松组与正常对照组比较差异无显著性。(3)肾脏病理学半定量分析显示各治疗组肾小管间质损伤总积分和肾小球硬化指数均明显低于模型组(P＜0．05或P＜0．01),病变较模型组均显著减轻。结论狼疮方具有一定的免疫抑制作用,可能通过干扰CD40信号,减少自身抗体产生,从而对慢性GVHD狼疮样肾炎小鼠模型具有一定的防治作用。Objective To explore the efficacy and the mechanism of Lupus Recipe (LR) in treating mice with lupus nephritis (LN).Methods Model of LN mice with chronic graft versus host disease (GVHD) was set up.Serum anti-ds-DAN antibody,expression of CD40 in splenocytes,and kidney function were tested after treat- ment by LR and prednisone,respectively.Results (1)Serum anti-ds-DAN antibody in the model group was sig- nificant different when compared with that in the normal control group(P＜0.01).Compared with the normal...

关 键 词：移植物抗宿主病 狼疮肾炎 狼疮方 CD40信号 

分 类 号：R285[医药卫生—中药学]