噬菌体突变库技术在蛋白质改造中的应用  

Construction and application of phage displayed mutagenesis protein library

作　　者：刘北一 富宁[1] 

机构地区：[1]第一军医大学基础部免疫学教研室,广东广州510515

出　　处：《免疫学杂志》2004年第z1期56-58,共3页Immunological Journal

基　　金：国家自然科学基金海外基金(30028021);广州市科技计划(2002E3-E0421)资助项目

摘　　要：噬菌体展示突变蛋白库是以噬菌粒为载体,对蛋白质序列中的位点(特异或随机)在DNA水平上进行突变而构建的次级文库。突变蛋白库构建策略包括:寡核苷酸介导的直接位点突变、盒式突变、DNA shuffling以及错倾PCR。蛋白突变库主要应用于提高蛋白亲和力、延长半衰期、确定蛋白关键残基。Phage displayed mutagenesis protein library is the second-generation library which constructed by mutating the site (specially or randomly) in the protein sequence at the DNA level and cloning in the phagemid vector. The construction strategy of mutagenesis protein library includes oligonucleotide-mediated site-directed mutagenesis, cassette mutagenesis, DNA shuffling, and error-prone PCR. Mutagenesis library is developed for identifying key residues of protein, raising the affinity, and prolonging half-life of protein.

关 键 词：噬菌体展示技术 突变库 关键残基 

分 类 号：Q819[生物学—生物工程]