FTIR方法研究若干味中药对SMMC-7721肝癌细胞的作用  

Effects of several Chinese herbal medicines on SMMC-7721 liver cancer cells by FTIR spectroscopy

作　　者：陆峰 陆国浩 曹永兵 肖振宇 吴玉田 

机构地区：[1]第二军医大学药学院药物分析学教研室,上海200433 [2]第二军医大学药学院药理学教研室,上海200433

出　　处：《第二军医大学学报》2004年第10期1100-1103,共4页Academic Journal of Second Military Medical University

基　　金：国家自然科学基金 ( 3 0 10 0 2 3 3 )

摘　　要：目的 :应用红外光谱法研究多味中药混合水提液对肝癌细胞的作用机制。 方法 :用傅立叶变换红外光谱法 (FTIR)研究肝癌 SMMC- 772 1细胞 (空白组 )及其经夏枯草、鱼腥草、柴胡、茵陈等 4味抗癌中药水提液培养 (中药组 ) 2 0 h后的红外光谱 ,考察光谱上表征蛋白质的酰胺带、核酸的磷酸二酯带等的峰形、峰位、峰强变化 ,以及部分特征峰峰强比的变化 ,并对各红外光谱进行聚类分析。 结果 :中药组与空白组的红外光谱在峰形、峰位、峰强度等均存在不同程度的差异 ,中药组的 υs(PO2 - )、υas(PO2 - )谱带多向低波数移动 ,υas(CH3)、δ(CH2 )谱带多向高波数移动。中药组的υas(CH3)与υs(CH2 )相对峰强比减小 ,υs(PO2 - )与 δ(N- H)相对峰面积比值大多升高 ,分别提示对 SMMU- 772 1细胞中膜结构的破坏作用、对 DNA复制的抑制作用等。聚类分析将中药组与空白组分开 ,不归肝经的中药与归肝经的中药分开。结论 :通过 FTIR可以对由中药作用引起的细胞变化进行快速、有效的分析 ,对阐明中药的作用机制有提示作用。Objective:To study the effects of several herbal medicines on SMMC-7721 liver cancer cells with Fourier transform infrared spectrometer(FTIR). Methods: FTIR was employed to determine the infrared spectra(IRs) of SMMC-7721 liver cancer cells cultivated for 20 h with the extracts of Spica prunellae, Herba houttuyniae, Radix bupleuri and Herba artemisiae scopariae. Cluster analysis of IRs was also performed. Results: IR spectral parameters such as band shape, intensity and frequency of the blank, control and herbal-extract-treated cells were compared. There existed obvious blue shift of υ s(PO 2 -), υ as (PO 2 -) bands, red shift of υ as (CH 3), δ(CH 2) bands on the herbal-extract-treated cells IRs. The decreasing ratio of υ as (CH 3) to υ s(CH 2) peak intensity and the increasing ratio of υ s(PO 2 -) to δ(N-H) peak area indicated the destructive effect of herbal extracts on the membrane structure of SMMU-7721 cells and inhibitory effect on the DNA replication respectively. Cluster analysis successfully discriminated the herbal-extract-treated cells from the blank cells and the liver-oriented medicines from the non-liver-oriented medicine. Conclusion: FTIR provides another fast and effective approach to analyze the changes of cells treated with Chinese herbal medicines, which may help to illuminate the functional mechanism of Chinese herbal medicines.

关 键 词：傅立叶变换红外光谱法 肝肿瘤 SMMC-7721细胞 中草药 

分 类 号：R286.91[医药卫生—中药学] R735.7[医药卫生—中医学]