5-脂氧合酶的分子生物学研究  

Molecular biology of 5-lipoxygenase

作　　者：刘宏[1] 周斌 陈新生 

机构地区：[1]第二军医大学长海医院口腔科,上海200433 [2]药学院药理学教研室,上海200433

出　　处：《第二军医大学学报》2004年第10期1124-1126,共3页Academic Journal of Second Military Medical University

基　　金：国家自然科学基金 ( 3 0 2 0 0 3 16)

摘　　要：5-脂氧合酶是合成白三烯的关键酶 ,白三烯是体内一种重要的炎症和过敏介质。采用同源重组方式敲除胚胎干细胞中的 5 -脂氧合酶基因 ,小鼠能正常生长和发育 ,但对某些炎症损伤的反应性与正常小鼠不同。5 -脂氧合酶在不同的细胞中表现出不同的亚细胞定位 ,分布于胞质或胞核 ,或两者都有。在一定条件下 ,5 -脂氧合酶发生胞核内外的穿梭 ,提示 5 -脂氧合酶可能还具有传统酶催化作用以外的其他功能。5-lipoxygenase (5LO) is the key enzyme in the synthesis of leukotrienes, which are potent biological mediators of inflammation and anaphylaxis.After lock out of the 5LO gene by homologous recombination in embryonic stem cells, 5LO deficient-mice can develop normally and stay healthy, with a selective opposition to certain inflammatory insults. The cellular distribution of 5LO in unstimulated cells among different cell types is different; it can present in the cytosol, the nucleus, or both. 5LO was also found capable of shuttling in and out of the nucleus under certain conditions. It is suggested that 5LO might have other functions besides its traditional catalytic role.

关 键 词：白三烯 5-脂氧合酶 核定位序列 转位 

分 类 号：Q559[生物学—生物化学]