基因扩增法快速检测阴沟肠杆菌Amp C β-内酰胺酶  

Rapid detection of Amp C β-Iactamase in Enterobacter cloacae by PCR

作　　者：赵虎[1] 何铭君[1] 张时良 

机构地区：[1]第二军医大学长征医院实验诊断科,上海200003 [2]江苏大学医学技术学院,镇江212001

出　　处：《第二军医大学学报》2004年第12期1329-1331,共3页Academic Journal of Second Military Medical University

基　　金：上海市启明星计划(QMX01423)

摘　　要：目的:建立检测阴沟肠杆菌Amp C β-内酰胺酶(简称Amp C酶),尤其是持续高产Amp C酶的快速方法。方法:合成阴沟肠杆菌amp C和amp D引物,扩增标准及临床分离的阴沟肠杆菌中amp C和amp D的基因片段,判断待检阴沟肠杆菌是否产Amp C酶,尤其是否产持续高产Amp C酶,并用头孢西丁三维试验加以证实。结果:临床分离的214株阴沟肠杆菌中193株amp C基因扩增阳性,其中amp D扩增阴性者为16株;头孢西丁三维试验阳性18株,其中16株amp C基因扩增阳性而amp D扩增阴性,男有2株amp C和amp D均阳性。去阻遏持续高产型标准菌株amp C扩增阳性、amp D扩增阴性,野生型标准菌株amp C和amp D均扩增阴性。结论:用amp C和amp D引物扩增法可检出18株持续高产Amp C酶的阴沟肠杆菌中的16株,阳性率为88.89％。该方法可用于检测阴沟肠杆菌是否产持续高产Amp C酶。Objective:To establish a new method to detect Amp Cβ-lactamase by PCR. Methods:The amp C and amp D gene fragments of E. cloacae were amplified by the amp C and amp D primers to detect Amp C β-lactamase, especially the enduring and highly productive enzymes. Results:Totally 193 of 214 strains of E. cloacae were positive for amp C gene , implicating most strains of E. cloacae had the ability to produce the enzyme. Sixteen of the 193 strains (amp C positive) were negative for amp D genes, implicating these 16 strains could produce the enduring and highly productive enzymes. The results were confirmed by cefoxitin three-dimensional test. Conclusion:The new method to detect Amp C β-lactamase, especially the enduring and highly productive enzymes,by amp C and amp D primers is a rapid and accurate method.

关 键 词：AMP C Β-内酰胺酶 PCR 阴沟肠杆菌 

分 类 号：R978.2[医药卫生—药品]