克雷伯杆菌微胶囊化生产1,3-丙二醇的研究  

Study on 1,3-propanediol production by immobilized Klebsiella pneumoniae in microcapsules

作　　者：陈宏文[1,2] 陈瑜丽 兰凤英 方柏山[2] 胡宗定[1] 

机构地区：[1]天津大学化工学院生化工程系,天津300072 [2]华侨大学生物工程与技术系

出　　处：《现代化工》2004年第z1期138-140,143,共4页Modern Chemical Industry

基　　金：国家自然科学基金(20276026);福建省自然科学重点项目基金资助(D0120002)

摘　　要：研究了用高压微胶囊成型装置制备克雷伯杆菌 NaCS-PDMDAAC 微胶囊生产1,3-丙二醇。考察了 PDMDAAC 浓度对微胶囊性能的影响及推进速度、电压、针头内径对微胶囊直径的影响。摇瓶条件下,微胶囊细胞连续发酵10批,粒径2.5mm 的微囊得到最大1,3-丙二醇浓度为13.38g/L,发酵时间从游离培养的34 h 缩短为19h,最大生产能力为0.60g/(L·h),比游离细胞提高2.56倍。The production of 1,3-propanediol by Klebsiella pneumoniae in NaCS-PDMDAAC microcapsules prepared in high-voltage electrostatic field was studied.The influence of PDMDAAC concentration on gel bead characteristics and its push speed,type of pinhead and voltage on microcapsule diameter were studied also.The encapsulated cells were fermented sequen- tially in 10 batches in shaking flasks,the maximum concentration of 1,3-propanediol was 13.38 g/L when the mean diameter of microcapsules was 2.5mm.The maximum 1,3-propanediol productivity was 0.60 g/(L·h),which was 2.56 times higher than that of flee cells.

关 键 词：1 3-丙二醇 NaCS-PDMDAAC微胶囊 固定化 

分 类 号：TQ223.162[化学工程—有机化工]