五灵胶囊药效作用的有效成分  

Test study of effective pharmacodynamic components in WuLing capsules

作　　者：王胜春[1] 胡咏武[1] 王俊琴[1] 李晓玮[1] 贺雪梅[1] 

机构地区：[1]第四军医大学西京医院药剂科,陕西西安710033

出　　处：《第四军医大学学报》2004年第17期1587-1591,共5页Journal of the Fourth Military Medical University

摘　　要：目的 :探讨五灵胶囊产生药效作用的活性成分 .方法 :分离肝细胞并建立肝细胞损伤模型 ,血清药效学试验证实五灵胶囊药效作用 ,HPLC色谱和紫外光谱分析含药血清中五灵胶囊化学成分 ,同时采用细胞药效学试验观察 4种对照品的药效作用 .结果 :模型组培养上清ALT ,AST ,MAO ,MDA酶活性分别升高至 (881 .8± 2 31 .7)nkat/L ,(1 2 5 6 .9± 2 6 5 .1 )nkat/L ,(32 .3± 2 .3)nkat/L ,(1 .0± 0 .6 )μmol/L ,与正常组比较 (P <0 .0 5 ) ;五灵胶囊含药血清能有效地拮抗D GlaN对肝细胞损伤 ,细胞上清液中ALT ,AST ,MAO ,MDA酶活性分别降低至 (4 2 1 .8± 2 88.4 )nkat/L ,(980 .2± 1 1 3.4 )nkat/L ,(2 1 .7± 5 .5 )nkat/L ,(1 .5± 1 .0 )μmol/L ,显著低于模型组 (P <0 .0 5 ) ;4种对照品能明显降低由D GlaN诱导肝细胞损伤时所致升高的酶谱活性水平 ,对D GlaN损伤的肝细胞有显著的修复和保护作用 .结论 :醇甲、乙素。AIM: To investigate the active components producing the pharmacodynamic effects in WuLing Capsules. METHODS: Liver cells were separated and the model of liver cell injuries was established. The pharmacodynamic role of WuLing capsules was demonstrated by pharmacodynamic test. The chemical components of WuLing capsules were analyzed by HPLC chromatograms and UV spectrum, and the pharmacodynamic effects of four kinds of control substance were observed by the test of cell pharmacodynamics. RESULTS: The D GlaN group active enzyme of ALT, AST, MAO and MDA rose respectively to (881.8±231.7) nkat/L, (1256.9±265.1) nkat/L, (32.3±2.3) nkat/L and (1.0±0.6) μmol/L, which were more marked than those in normal group ( P <0.05). The drug serum in WuLing capsules antagonized the injuries of liver cell induced by D GlaN and the enzyme activeness of ALT, AST, MAO and MDA decreased respectively to (421.8±288.4) nkat/L, (980.2±113.4) nkat/L, (21.7±5.5) nkat/L and (1.5±1.0) μmol/L, notably lower than those in model group ( P <0.05). Four kinds of control substance markedly decreased the level of high enzyme activity that was induced by D GlaN and there were obvious action of restoration and protection on the injuries of liver cells induced by D GlaN . CONCLUSION: Schizandrol A, Schisandrin B, Crytanshinone and Tanshinone ⅡA are effective components of WuLing capsules in retarding the injuries of liver cells.

关 键 词：五灵胶囊 肝损伤 活性成分 

分 类 号：R285.6[医药卫生—中药学]