annexinⅡ在人表皮角质形成细胞分化中的表达  

Expression of annexinⅡ in differentiation of human epidermal keratinocyte

作　　者：李习玲[1] 连小华[1] 张诚[1] 杨恬[1] 

机构地区：[1]第三军医大学细胞生物学教研室,重庆400038

出　　处：《第四军医大学学报》2004年第21期1921-1924,共4页Journal of the Fourth Military Medical University

基　　金：国家自然科学基金 (30 371 383)

摘　　要：目的 :探讨annexinⅡ在正常人表皮角质形成细胞(normalhumanepidermalkeratinocytes,NHEK)分化中的表达 .方法 :①体外培养NHEK ,采用无血清无钙离子的角质形成细胞生长培养基 (KGM) ,通过改变培养基中的钙离子浓度来调节NHEK的增殖和分化 ,采用免疫细胞化学 (ICC)技术分别检测annexinⅡ在增殖和分化NHEK中的表达 .②采用免疫组织化学 (IHC)技术检测成人及新生儿表皮NHEK中annexinⅡ蛋白的表达 .结果 :①低钙 (0 .0 7mmol L)培养条件下 ,annexinⅡ呈弱阳性表达 ,且主要分布于核周 .高钙 (1.5mmol L)条件下培养 2 4h后 ,annexinⅡ仍呈弱阳性表达 ,但表达的部位出现明显变化 ,主要分布于细胞间 ;4 8h后 ,annexinⅡ于胞质及胞膜均呈强阳性表达 .②人正常皮肤切片的IHC结果显示 :annexinⅡ主要分布于基底上层的分化NHEK中 .结论 :annexinⅡ可能参与人表皮NHEK分化的调控 .AIM: To explore the expression of annexinⅡ in differentiation of human epidermal keratinocytes. METHODS: ① Normal human epidermal keratinocytes(NHEK)were cultured in serum and Ca 2+ free keratinocyte growth medium (KGM). The proliferation and differentiation of keratinocytes was regulated by changing Ca 2+ concentration in medium and the expression of annexinⅡ was detected by immunocytochemistry (ICC). ② Immunohistochemistry was used to detect annexinⅡexpression in noenatal and adult epidermis. RESULTS: ①When Ca 2+ concentration in medium was 0.07 mmol/L, annexinⅡ was expressed weakly and was located perinuclearly. When the calcium concentration was elevated to 1.5 mmol/L, the expression of annexinⅡ was still slight, but with a dramatic redistribution to the cell-cell borders within 24 h after the calcium elevation. Within 48 h after the calcium elevation, annexinⅡ was seen both in cytoplasm and cell membrane and had a much strong expression. ② In sections of human normal skin, annexinⅡ was mainly localized in the superficial and more differentiated layers of the epidermis. CONCLUSION: AnnexinⅡ may be involved in regulating the differentiation of NHEK.

关 键 词：annexinⅡ表达 表皮 角质形成细胞 细胞分化 

分 类 号：Q28[生物学—细胞生物学]