Endonuclease G参与小鼠海马神经元的凋亡  

Endonuclease G is involved in neuronal apoptosis in mouse hippocampus

作　　者：武永飞[1] 张建华[2] 李生斌[1] 

机构地区：[1]西安交通大学法医学国家重点实验室,陕西西安710061 [2]美国辛辛那提大学医学院细胞生物学神经生物学与解剖学系

出　　处：《第四军医大学学报》2004年第21期1925-1927,共3页Journal of the Fourth Military Medical University

基　　金：国家留学基金委员会访问学者基金 (2 2 861 0 1 0 ) ;NIH(DA1 1 2 84)

摘　　要：目的 :研究Endonuclease (EndoG)是否参与由兴奋性氨基酸所诱导的神经细胞凋亡 .方法 :用Westernblotting法检测EndoG+ - 小鼠及EndoG+ + 小鼠海马结构中EndoG的表达 .用KA诱导抽搐反应及神经细胞凋亡 .用TUNEL法分析En doG+ - 小鼠及EndoG+ + 小鼠海马结构中神经细胞凋亡的情况 .结果 :EndoG在EndoG+ - 小鼠海马结构中的表达比在En doG+ + 小鼠中降低 4 0 %以上 .KA作用后 ,EndoG+ - 小鼠与EndoG+ + 小鼠表现出相同的神经兴奋性 .KA作用后 ,与En doG+ + 相比 ,EndoG+ - 小鼠海马结构CA3,CA1亚区细胞凋亡下降了约 35 % .结论 :EndoG参与了由兴奋性毒性所诱发的神经细胞凋亡 .AIM: To investigate the involvement of Endonuclease G (EndoG) in excitatory amino acids induced neuronal apoptosis. METHODS: EndoG expression in the hippocampus of EndoG +/+ mice and EndoG +/- mice was analyzed by Western blotting. Kainic acid (KA) was taken to induce seizure behavior and excitotoxicity in EndoG +/- mice and EndoG +/+ mice. The terminal deoxynucleotidyl transferase-mediated UTP nick end-labeling (TUNEL) assay was used to detect neuronal apoptosis in the hippocampus after KA treatment. RESULTS: We found that EndoG expression in the hippocampus of EndoG +/- mice was reduced more than 40% compared to that in EndoG +/+ mice. The reduction of EndoG expression levels in the hippocampus did not result in altered KA-induced seizure severity in EndoG +/- mice compared to that in EndoG +/+ mice. However, the KA-induced apoptotic cells in both CA3 and CA1 in EndoG +/- mice were reduced by about 35% in comparison with those in EndoG +/+ mice. CONCLUSION: The reduced expression of EndoG in the hippocampus of EndoG +/- mice leads to resistance to excitotoxicity-induced apoptosis.

关 键 词：脱氧核糖核酸酶BamHI 凋亡 兴奋性毒性 红藻氨酸 海马 

分 类 号：Q189-35[生物学—神经生物学]