低强度脉冲电磁场对大鼠骨密度及骨形态计量学的影响  

Effects of low intensity PEMFs on bone mineral density and histomorphometry in SD rats

作　　者：徐巧玲[1] 路丽华[2] 罗二平[3] 申广浩[3] 刘冬焕[1] 化前珍[1] 吴晓明[3] 李丽荣[3] 

机构地区：[1]第四军医大学护理系,陕西西安710033 [2]第四军医大学基础部化学教研室,陕西西安710033 [3]第四军医大学生物医学工程系军队卫生装备与计量学教研室,陕西西安710033

出　　处：《第四军医大学学报》2004年第21期2004-2006,共3页Journal of the Fourth Military Medical University

基　　金：陕西省自然科学基金 (2 0 1 2 0 0ZF33)

摘　　要：目的 :探讨低强度脉冲电磁场对大鼠骨密度及生物力学特性的影响 .方法 :将 32只 3mo龄雌性SD大鼠随机分为 4组 (n =8) :去势组、对照组、磁场A组、磁场B组 ;各组大鼠均采用下腹部切口 ,去势组及磁场组行双侧卵巢切除术 ,对照组仅行开腹术 .其中 ,磁场A组暴露于强度为 4× 10 - 4T的脉冲磁场环境中 ,磁场B组暴露于强度为 8× 10 - 4T的脉冲磁场环境中 ,两组磁场频率f均为 15Hz,τ为 5ms,暴磁时间为6h d .各组动物饲养条件相同 ,8wk后处死 ,测定全身骨密度及骨形态计量学指标 .结果 :与去势组大鼠相比 ,磁场组全身骨密度、股骨远端骨密度、骨小梁面积百分比、骨小梁宽度、骨小梁数目均有增加 (P <0 .0 5 ) ,骨小梁间隙减小 (P <0 .0 5 ) ;而与对照组相比 ,磁场B组全身骨密度、骨小梁面积百分比以及骨小梁数目等指标间的差异则没有显著性意义 (P >0 .0 5 ) ;两磁场组之间作比较 ,全身骨密度和骨小梁数目的差异显著(P <0 .0 5 ) .结论 :PEMFs对卵巢切除大鼠骨质疏松症的发生有一定的预防和治疗作用 ,磁场生物效应存在明显的窗口效应 .AIM: To investigate the effects of pulsed electromagnetic fields (PEMFs) on bone mineral density (BMD) and histomorphometry in ovariectomized rats. METHODS: Thirty-two female SD rats were divided randomly into 4 groups: ovariectomy, control, magnetic A, magnetic B. All the rats were operated on the abdomen and the rats of ovariectomy group and magnetic groups (A, B) were ovariectomized. The rats of control group only had sham operation and were not ovariectomized. The rats in both magnetic groups were exposed to 15 Hz PEMFs 6 hours per day, with its pulse duration set to 5 ms. Magnetic group A and group B were exposed to PEMFs with different magnetic intensity, 4×10 -4 T for group A and 8×10 -4 T for group B. All the animals were killed 8 weeks after operation and the changes of BMD and histomorphometric properties were observed. RESULTS: The values of BMD, trabecular area, trabecular thickness and trabecular number in ovariectomized group significantly decreased, compared with those of the other three groups(P<0.05), while the trabecular separation in ovariectomized group increased obviously, compared with magnetic group A and magnetic group B (P<0.05). Compared with those of control group, no significant differences were found in the values of total body BMD, trabecular area and trabecular number in magnetic group B (P>0.05). But, statistically significant differences were observed in the values of total body BMD and trabecular number between the two magnetic groups(P< 0.05) . CONCLUSION: There are some evident 'window-effects' during the treatment by PEMFs. PEMFs are effective in improving BMD and histomorphometric values and are favorable to the treatment and prevention of osteoporosis.

关 键 词：脉冲电磁场 骨质疏松 骨密度 骨形态计量学 

分 类 号：R644[医药卫生—外科学]