阿魏酸钠对血小板源生长因子和内皮素1诱导的血管平滑肌细胞迁移的影响  

Effect of Sodium Ferulate on Migration Induced by Platelet Derived Growth Factor and Endothelin-1 in Cultured Vascular Smooth Muscle Cells

作　　者：韩英[1] 谢良地[1] 许昌声[1] 王华军[1] 

机构地区：[1]福建省高血压研究所福建医科大学附属第一医院,福建省福州市350005

出　　处：《中国动脉硬化杂志》2004年第6期659-661,共3页Chinese Journal of Arteriosclerosis

基　　金：福建省医学创新课题 (C2 0 0 3 CX 12 )

摘　　要：为探讨阿魏酸钠对血小板源生长因子二聚体和内皮素 1诱导的血管平滑肌细胞迁移的影响 ,采用组织块外生法体外培养血管平滑肌细胞 ,采用改良的Boyden微孔膜双槽法进行细胞迁移实验 ,荧光染料Fura 2 /AM法测定细胞内游离钙离子浓度。结果发现 ,血小板源生长因子二聚体和内皮素 1均可诱导血管平滑肌细胞迁移 ,作用峰值浓度分别为 10 μg/L和 10 - 7mol/L。阿魏酸钠 (10 - 7～ 10 - 3mol/L)呈浓度依赖性抑制上述物质诱导的细胞迁移 ,10 - 3mol/L阿魏酸钠对血小板源生长因子二聚体和内皮素 1诱导的细胞迁移的抑制率分别为 85 .0 4 %和 81.92 %。血小板源生长因子二聚体和内皮素 1促进细胞内游离钙离子浓度升高 (P <0 .0 5 ) ,作用峰值浓度分别为 10μg/L和 10 - 8mol/L。阿魏酸钠明显抑制该作用 ,峰抑制率分别为 80 .14 %和 76 .6 9%。以上提示 ,阿魏酸钠可能通过抑制细胞内游离钙离子浓度的升高来抑制上述物质诱导的血管平滑肌细胞迁移。Aim To investigate the effect of sodium ferulate(SF), one of the principal components of rhizoma ligustici wallichii, on the migration induced by endothelin-1 (ET-1) and platelet derived growth factor (PDGF) in vascular smooth muscle cells(VSMC). Methods Cultured VSMC derived from spontaneously hypertensive rats(SHR) were used. Cell migration was determined by modified Boyden chamber assays. [Ca 2+]i was measured with fluorescent Ca 2+ indicator Fura-2/AM. Results ET-1 and PDGF significantly induced a migration of VSMC in a dose-dependent manner, which was inhibited by pre-treatment of VSMC with SF(10 -7～10 -3mol/L) dose-dependently. The peak inhibition rate of migration induced by ET-1 and PDGF were 85.04% and 81.92% (P<0.01) respectively. ET-1 and PDGF provoked the rise of [Ca 2+]i in VSMC which was significantly suppressed by 10 -3 mol/L SF(P<0.01)with a inhibitory peak at 80.14% and 76.69%. Conclusions The migration and rise of [Ca 2+]i induced by ET-1 and PDGF in VSMC from SHR may be suppressed by SF.

关 键 词：药理学 血小板源生长因子 内皮素1 细胞内游离钙离子 阿魏酸钠 血管平滑肌细胞 细胞迁移 

分 类 号：R96[医药卫生—药理学]