乙肝患者抗原抗体模式及病程与IL-16载量的关系  

The Relationship Between the Quantities of the Models of Serologic Markers and Period in Patients Infected with HBV and Contents of Interleukin-16 in serum

作　　者：罗春香[1] 杨志[1] 罗亚桐[1] 杨智[1] 罗向波 张静[3] 

机构地区：[1]湖南师范大学,中国湖南长沙410006 [2]长沙市传染病医院 [3]华中科技大学同济医学院妇幼系

出　　处：《实用预防医学》2004年第4期659-661,共3页Practical Preventive Medicine

基　　金：湖南省卫生厅资助课题 (编号 :Y0 2 - 0 84)

摘　　要：目的　探索乙型病毒性肝炎患者抗原抗体模式及病程与血清白细胞介素 -16(IL -16)的关系 ,为乙型肝炎的发病机理提供新的思路。　方法　用酶链免疫吸附 (ELISA)法检测血清IL -16水平及抗 -HBc -IgM、抗 -HBc -IgG;用酶免疫法 (EIA)检测血清HBsAg、抗 -HBs、HBeAg及抗 -HBe。　 结果　血清IL -16水平在a +c +e +(f)模式明显升高 (P <0 .0 1) ;病程小于 1月者 (P <0 .0 1)、病程 1-3月者 (P <0 .0 1)和病程 3 -6月者 (P <0 .0 5 )血清IL -16水平明显升高。　结论　血清IL -16在HBV感染早期病毒高水平复制期明显升高 ,提示IL -16可能参与HBV感染后急性期及重型肝炎的肝损伤过程 ,是一种潜在的早期促炎症反应细胞因子。Objective To investigate the correlation between the models of antigen/antibody,period in patients infected with HBV and the levels of interleukin-16 in serum so that we can provide a new thinking for pathogenesis of hepatitis B. Methods ELISA was applied to detect the levels of interleukin-16 in serum,anti-HBc-IgM and anti-HBc-IgG.EIA was used to determine HBsAg,anti-HBs,HBeAg and anti-HBe.Real-time fluorescent quantitative PCR was used to measure the HBV DNA. Results IL-16 in both the models of antigen/antibody and the course were much higher than those in healthy control group,respectively with statistic difference between a+c+e and a+c+e+f models,between less than one month and from one to three months or three to six months. P value were less than 0.01,0.01,and 0.05 respectively when being comparied with those of healthy control. Conclusion IL-16 level in serum was obviously high in both early hepatitis B and period with a good many HBV DNA virus reproduction,which showed that IL-16 might participate in the course of liver injury in acute and severe hepatitis B.It was found that IL-16 was a potential cytokine for promoting inflammation.

关 键 词：血清学标志 乙肝病毒 病程 白细胞介素-16 

分 类 号：R373.2+1[医药卫生—病原生物学]