液相色谱-电喷雾离子阱质谱法快速鉴定刺五加提取物中的四种组分  

Rapid Identification of Acanthopanax senticosus Mixture by Liquid Chromatography-Electrospray Ion Trap Mass Spectrometry

作　　者：邢杰[1] 张淑秋[1] 钟大放[1] 贾继明 

机构地区：[1]沈阳药科大学药物代谢与药物动力学实验室,辽宁沈阳110016 [2]哈尔滨中药二厂,黑龙江哈尔滨150001

出　　处：《质谱学报》2004年第4期198-203,共6页Journal of Chinese Mass Spectrometry Society

基　　金：国家自然科学基金资助项目 (39930 180 )

摘　　要：探讨了采用液相色谱 -电喷雾串联质谱法 ( L C-MSn,n=1～ 3 )检测刺五加中异秦皮定以及微量双环木脂素松脂醇 -二 -O-β-D-葡萄糖苷、刺五加苷 D和松脂醇的方法。流动相为甲醇 -水 ( 5 0∶ 5 0 ,体积比 )的混合液。色谱柱为 Diamonsil C18(内径 2 0 0 mm× 4.6mm,粒径 5μm) ,流速为 0 .5 m L/min。结果表明 :异秦皮定、松脂醇 -二 -O-β-D-葡萄糖苷、刺五加苷 D和松脂醇的准分子离子 ( [M+H]+ )和它们的 MS2 主碎片离子分别为 m/z 2 2 3、m/z 70 0、m/z 2 0 8、m/z 2 3 5、m/z 41 9和 m/z 2 3 5。The method of reversed-phase high performance liquid chromatography-electrospray ionization tandem mass spectrometry (LC-ESI/MS n, n=1-3) for the rapid identification of isofraxidin and trace lignans, pinoresinol-di-O-β-D-glucoside, eleutherosides D and pinoresinol, in Acanthopananx senticosus Harms was established. The mixtures were successfully isolated on a Diamonsil C 18 column (200mm×4.6mm×5μm), using methanol-water (50∶50, v/v) as the mobile phase at a flow rate of 0.5mL/min. Compared with the LC-ESI-MS n spectra of standard samples, isofraxidin and three lignans in Acanthopanax senticosus mixture were identified. The results showed the quasimolecular ion and MS 2 main fragment ion peaks of isofraxidin, pinoresinol-di-O-β-D-glucoside, eleutherosides D and pinoresinol are m/z223([M(222)+H] +), m/z700([M(682)+NH 4] +), m/z760([M(742)+NH 4] +) and m/z359([M(358)+H] +), and m/z208, m/z235, m/z419 and m/z235, respectively.

关 键 词：刺五加提取物 液相色谱-电喷雾离子阱质谱 快速分析 

分 类 号：O657.63[理学—分析化学] Q949.7632[理学—化学]