葡萄卷叶病毒RT-PCR检测技术研究  

Studies on RT-PCR Detection Technology of GLRaV Ⅲ

作　　者：牛建新[1] 陈萍[1] 李西平[1] 马兵钢[1] 鲁晓燕[1] 

机构地区：[1]石河子大学农学院园艺系

出　　处：《西北农业学报》2004年第1期28-32,共5页Acta Agriculturae Boreali-occidentalis Sinica

基　　金：国家自然科学基金资助项目(30060053);兵团科委项目(NKB02SDXNKO1SW)。

摘　　要：以葡萄叶片和皮层为材料,对植物总RNA和dsRNA提取方法进行了研究,从中筛选出了提取葡萄总RNA和dsRNA的适合方法。建立了GLRaV RT-PCR的有效体系,在有效体系基础上,通过研究RT-PCR主要成分对RT-PCR的影响,确立了GLRaVRT-PCR的优化体系。Using grapevine leaves and cortexes as materials, total RNA was extracted using three methods ,and compared these methods in these paper. The fittest methods for Grapevine were screened out. PCR amplification conditions were optimized. Based on the effectual RT-PCR detection system of Grapevine leafroll virus Ⅲ. Concentration of RT reaction and PCR reaction primary ingredients were optimized and decrease correctly.

关 键 词：葡萄卷叶病毒Ⅲ RT-PCR检测 

分 类 号：S436.63[农业科学—农业昆虫与害虫防治]