中药912液防治大鼠内毒素所致急性DIC的实验研究  

Experhnental Study on Therapeutic and Preventive Effect of Compound 912 on Endotoxin-Induced DIC in Rats

作　　者：王红[1] 张淑文[1] 赵立业[1] 王宝恩[1] 魏东[2] 刘飒[2] 张叔伦[2] 

机构地区：[1]首都医科大学附属北京友谊医院感染内科,北京100050 [2]北京首都儿科研究所病理生理研究室

出　　处：《中国中西医结合杂志》2004年第S1期265-268,共4页Chinese Journal of Integrated Traditional and Western Medicine

摘　　要：目的观察 DIC 形成过程中活体肠系膜微循环状态的病理改变以及912液对其影响。方法将大鼠分为4组:对照组、内毒素组、中药预防组、中药治疗组。间隔24h 两次注入内毒素,于第2次注入内毒素后,暴露肠系膜,持续观察2～3h。静脉推注吖啶橙,以落射的紫外线激发荧光,清晰地显示血管内皮、白细胞、血小板和白细胞血栓。结果在内毒素组大鼠肠系膜微小静脉中可以观察到荧光标记的白细胞贴壁、聚集和白细胞血栓,并可见到血流减慢、停滞及微血流流态的改变。组织病理学检查,可见肾、肺组织内的微血栓。中药预防组可见内毒素引起的白细胞贴壁、聚集现象明显减轻,无血流流速的减慢和停滞。中药治疗组,可见黏附、聚集的白细胞脱落,已形成的白细胞血栓部分溶解脱落,并使已停滞的微血管血流具有重新恢复的趋势。结论 (1)用 Schwartzman 现象的原理,以静脉推注内毒素诱发,可成功地制成实验性 DIC 的动物模型。(2)本模型显示如下的肠系膜微循环病理改变:毛细血管内白细胞黏附、聚集及白细胞血栓形成;血流流速减慢及血流停滞,流态呈粒流、粒摆流;病理组织学检查,可见肺、肾组织中有瘀血、血流停滞、白细胞聚集及弥漫性纤维蛋白血栓的形成及继发出血。(3)中药912液有预防及治疗实验性 DIC 的作用。Objective To investigate the effect of compound 912 on endotoxin-induced DIC in rats.Methods Thirty rats were divided into 4 groups,the control group,endotoxin group,prevention group,and compound 912 treating group. Each rat was given endotoxin twice between 24 hours.The mesenteric microcirculation was observed for 2—3 hours suc- cessively after the second time endotoxin was given with microscopy which was connected with camera record.The blood vessel endothelium,behavier of leukocyte and leukocyte microthr...

关 键 词：弥漫性血管内凝血 内毒素血症 微循环 

分 类 号：R285.5[医药卫生—中药学]