青心酮对活化RAW264.7细胞株血红素氧合酶-1mRNA/一氧化碳及TNF-α表达的影响  

Effect of DHAP on Expression of Hemeoxygenase-mRNA/Carbon Monoxide and TNF-αin Activated RAW 264.7Cell Line

作　　者：张代娟[1] 吴萍[1] 盘茜[1] 万敬员[1] 张力[1] 吴涛[1] 周晓燕[1] 叶笃筠[1] 

机构地区：[1]华中科技大学同济医学院病理生理教研室,武汉430030

出　　处：《中国中西医结合杂志》2004年第S1期257-260,共4页Chinese Journal of Integrated Traditional and Western Medicine

基　　金：国家自然科学基金资助课题(No.30100226)

摘　　要：目的观察青心酮对活化 RAW264.7细胞株血红素氧合酶-1mRNA/一氧化碳(Hemeoxygenase-1/carbon monoxide,HO-1mRNA/CO)及肿瘤坏死因子-α(TNF-α)表达的影响。方法用 LPS 做刺激剂,建立活化细胞模型。分别用 RT-PCR 法检测 HO-1mRNA 的表达,血红蛋白结合法检测 CO 的相对含量,Western-blot 方法检测 TNF-α蛋白质的表达。结果 10^(-5)mol/L 青心酮使活化巨噬细胞 HO-1mRNA 表达增加,CO 增加,TNF-α蛋白表达下降。结论青心酮上调活化 RAW264.7细胞株 HO-1mRNA/CO 的表达,抑制 TNF-α蛋白的产生,这可能是 DHAP 抗炎作用机制之一。Objective To observe effect of DHAP on expression of hemeoxygenase-mRNA/carbon monoxide and tumor necrosis factor α(TNF-α)in activated RAW 264.7 cell line.Methods Activated cell model was established by using IPS as stimulant.Expression of HO-1mRNA was determined by RT-PCR method.Relative content of CO was determined by hemoglobin binding test;expression of TNF-αprotein by Western-blot method.Results DHAP of 10^(-5) mol/I,could in- crease HO-1mRNA expression and CO content,decrease TNF-αprotein.Conclusion D...

关 键 词：青心酮 RAW264.7细胞株 血红素氧合酶-1 一氧化碳 肿瘤坏死因子-Α 

分 类 号：R285.5[医药卫生—中药学]