髓清丸对内皮细胞增殖影响的血清药理学研究  

Sero-pharmacological Study for Effect of Suiqing Pill on Endothelial Cell Proliferation

作　　者：杨振江[1] 赵霞[1] 刘莹[1] 欧阳郴生[1] 古宏晖[1] 戴玲[1] 

机构地区：[1]深圳市中医院,广东518033

出　　处：《中国中西医结合杂志》2004年第S1期252-254,共3页Chinese Journal of Integrated Traditional and Western Medicine

基　　金：广东省中医药管理局科研课题(No.98107)

摘　　要：目的研究中药复方髓清丸是否对内皮细胞的增殖有影响。方法采用血清药理学方法,以含髓清丸药物兔血清,体外培养观察内皮细胞系 ECV304的增殖、细胞周期及 DNA 含量等细胞学指标变化。结果与正常胎牛血清对照组和兔血清对照组比较,含髓清丸低、高剂量组的活细胞百分率、活细胞浓度均降低(P<0.05,P<0.01);含髓清丸血清低、高剂量组的培养液孵育的内皮细胞 OD 值呈不同程度的降低(P<0.01),高剂量组细胞毒活性>20%;髓清丸高、低剂量组内皮细胞 G_1期百分率均明显高于胎牛血清和正常兔血清组(P<0.01),而 S 期和 G_2期百分率也均较胎牛血清组和正常兔血清组有减低趋势。结论髓清丸体外有一定的抑制内皮细胞增殖的活性。Objective To probe the effect of Suiqing pill(SQP),a Chinese compound recipe,on endothelial cell prolifera- tion.Methods Adopting the sero-pharmacological method,the effects of SQP contained serum on proliferation,cell cy- cle and DNA content of cultured endothelial cell series ECV304 were observed in vitro.Results After treatment by SQP serum,high or low dose,the live cell percentage and concentration,OD value of endothelial cells in culture fluid were lower than those after treated by normal fetal bovine ...

关 键 词：髓清丸 内皮细胞 增殖 药物血清 

分 类 号：R285.5[医药卫生—中药学]