Akt信号通路在左归丸对体外培养新生大鼠海马神经干细胞凋亡中的作用  

Function of Akt Signal Pathway on Apoptosis of Hippocampus Neural Stem Cells of Newborn Rats Treated with Zuogui Pill in vitro

作　　者：高唱[1] 王景周[1] 洪局 

机构地区：[1]第三军医大学大坪医院野战外科研究所神经内科,重庆400042

出　　处：《中国中西医结合杂志》2004年第S1期62-65,共4页Chinese Journal of Integrated Traditional and Western Medicine

基　　金：国家自然科学基金资助(No.30271652)

摘　　要：目的观察 Akt 信号通路在左旋单钠谷氨酸(MSG)和左归丸(ZGW)对新生大鼠体外培养海马神经干细胞(NSC)和子细胞凋亡中的作用。方法分离、培养新生大鼠海马 NSC,选第二代克隆球做诱导分化实验。每个培养孔(3ml 培养体积)加入终浓度为10μmol//L 的 MSC 或10μmol/L MSG 加左归丸10μg 与NSC 孵育30min,培养7天后收集细胞进行检测。采用流式细胞仪和 Western blot 方法分别检测细胞凋亡率和 Akt 信号通路变化。结果 MSG 可增加细胞凋亡率,抑制总 Akt 表达,显著降低 Akt(Ser473)和 Akt(Thr308)水平;明显增加 FKHR(Ser256)、GSK-3β(Ser9)和 PTEN 表达。ZGW 可抑制 MSG 的部分效应。分别给予 PI3K选择性抑制剂 LY294002和激动剂,可显著反转和增强 ZGW 效应。结论左归丸能抑制 NSC 及其子细胞凋亡,这一作用与 Akt 信号通路有关。Objective To observe the function of Akt signal pathway on apoptosis of hippocampus neural stem cell (NSC) and daughter-cells of newborn rats treated with MSG and Zuogui Pill (ZGP).Methods Hippocampus NSC of newborn rats were differentiated and cultured.Induced differentiation experiment was done on clone ball of the second generation.End concentration of 10 μmol/L MSG,or 10 μmol/L MSG+10μg of ZGP was added to every culture pore (culture volume of 3 ml)and incubated for 30 min.Cells were collected to be tes...

关 键 词：左归丸 神经干细胞 凋亡 AKT 信号通路 

分 类 号：R285.5[医药卫生—中药学]