新疆出血热病毒单克隆抗体的应用研究:反向被动血凝和血凝抑制试验  

Study on Application of Monoclonal Antibody Against XHF Virus in RPHA and RPHI Tests

作　　者：冯崇慧[1] 王冬莉[1] 刘宏斌[1] 顾媛[1] 白旭华[1] 

机构地区：[1]新疆维吾尔自治区卫生防疫站,新疆乌鲁木齐830011

出　　处：《地方病通报》2004年第S1期-,共3页Endemic Diseases Bulletin

摘　　要：使用6株抗新疆出血热病毒单克隆抗体(单抗)致敏醛化绵羊红血球,以反向被动血凝试验(RPHA)检测病毒抗原;以反向血凝抑制试验(RPHI)检测人和各种动物血清抗体。结果6株单抗致敏血球在检测病毒抗原中以IgM型的14B7单抗的灵敏度最高。比其他5个单抗高16~32倍,而且与正常对照抗原完全没有非特异性凝集,远超过多克隆抗体致敏的血球。用RPHI检测血清抗体中,以13C6和43E5两个单抗的灵敏度高。测定抗体滴度的终点远高于多克隆抗体致敏的血球。RPHA可用于急性期病人血液中病毒抗原的检测,有很好的早期诊断价值,还可用于新分离病毒的血清学鉴定。RPHI可用于人和动物血清抗体的检测。已制出冻干的制剂,可保存1年以上,对于基层和现场防治调查工作非常方便。The monoclonal antibodies secreted by 6 strains of hybridoma against Xinjiang hemorrhagic fever virus, were used to sensitize the sheep erythrocytes for virus antigen detection with RPHA and antibody detection with RPHI test. The IgM type McAb secreted by 14B_7 hybridoma had more sensitivities in detection of virus antigen and no nonspecific agglutination was found. It was valuable in early diagnosis and to be used in identification of new isolated virus strains. The IgG1 type McAb of 13C_6 and 43E_5 were b...

关 键 词：反向被动血凝 反向被动血凝抑制 新疆出血热 单克隆抗体 

分 类 号：R392[医药卫生—免疫学]