PP_(333)、B_9和CCC对脱毒马铃薯试管繁殖的影响  

Effects of PP_(333), B_9 and CCC on in vitro Propagation of Virus-free Potato

作　　者：赵建萍[1] 蒋小满[1] 柏新富[1] 刘小莉[1] 武玉永[1] 

机构地区：[1]烟台师范学院生物科学与技术系,烟台264025

出　　处：《植物生理学通讯》2003年第6期571-574,共4页Plant Physiology Communications

摘　　要：以MS0 为增殖培养基 ,1/ 2MS(大量元素减半 ) +6 BA 0 .1mg·L-1+NAA 0 .1mg·L-1为生根培养基 ,分别附加不同浓度PP3 3 3 、B9和CCC ,结果表明 :2 0mg·L-1B9或 2 0～ 5 0mg·L-1CCC对脱毒马铃薯试管苗兼有促进增殖和复壮的双重效果 ;0 .0 5～ 0 .5mg·L-1PP3 3 3 及 5 0～ 10 0mg·L-1CCC适合于试管苗的保存 ;而 0 .0 5～ 0 .1mg·L-1PP3 3 3 及 2 0～ 10 0mg·L-1CCC有利于根的发生及移栽 ,其中尤以 0 .1mg·L-1PP3 3 3 效果最佳 ,其试管苗生根率和移栽成活率均为 10 0 % ,且移栽后缓苗期短 。To test the effects of PP 333, B 9 and CCC on in vitro propagation of the virus-free potato, we added them at different concentrations respectively and separately to both the base media for shoot multiplying (MS 0) and the base media for rooting (1/2MS+ 6-BA 0.1 mg L -1+NAA 0.1 mg·L -1), in which the shoots of the virus-free potato were cultured. The results showed that B 9 (20 mg·L -1 )or CCC ( 20～50 mg·L -1) could promote the proliferation and rejuvenation of the tube-plantlets of the virus-free potato; PP 333 (0.05～0.5 mg·L -1) or CCC (50～100 mg·L -1) was suitable for the preservation of the tube-plantlets; PP 333 (0.05～0.1 mg·L -1)or CCC (20～100 mg·L -1) was advantageous rooting and transplanting, and among them PP 333( 0.1 mg·L -1) was most effective. The rooting rate and survival rate after the transplantation were 100%. The redartant growth of seedlings was short and the plants grew vigorously.

关 键 词：脱毒马铃薯 试管繁殖 多效唑(PP333) 比久(B9) 矮壮素(CCC) 

分 类 号：S532[农业科学—作物学]