硝酸银和乙烯利对黄瓜叶片中3种氧化还原酶同工酶和酶活性影响的比较  

Comparative Study on the Effects of Silver Nitrate and Ethephon Leave Application on Three Oxidoreductase Isozymes and Their Activities in Cucumber (Cucumis sativus L.)

作　　者：娄群峰[1] 陈劲枫[1] 任刚[1] 罗向东[1] 

机构地区：[1]南京农业大学园艺学院作物遗传与种质创新国家重点实验室,南京210095

出　　处：《植物生理学通讯》2003年第6期580-584,共5页Plant Physiology Communications

基　　金：国家自然科学基金 (No .3 0 170 64 4);上海市科技兴农重点攻关项目 ( 2 0 0 1 3 3 );国家高技术研究发展计划专项经费(Nos .2 0 0 1AA2 4112 3 ;2 0 0 2AA2 412 5 1;2 0 0 2AA2 0 70 12 );教育部"跨世纪优秀人才培养计划";教育部科学技术研究重点项目 (重点 0

摘　　要：黄瓜经 30 0mg·L-1硝酸银和 2 0 0mg·L-1乙烯利处理后 ,叶片中过氧化物酶 (POD)和超氧化物歧化酶 (SOD)两种同工酶的酶谱带数和酶活性均明显受到诱导 ,而过氧化氢酶 (CAT)几乎不受影响。两种药剂处理的POD和SOD酶谱和活性变化有明显差异 ,硝酸银诱导两种酶带和活性高峰出现早 ,而乙烯利则能诱导更多的酶带数。The results indicated that, in terms of numbers of band and activities of peroxidase (POD) and superoxide dismutase (SOD) were significantly induced after treated with silver nitrate and ethephon at 300 mg·L -1 and 200 mg·L -1 in cucumber leaves, respectively. Significant differences were observed between the two treatments as well. Silver nitrate could induce the earlier peak in the expression of POD and SOD, ethephon could induce more bands of both enzymes. While catalase (CAT) was not induced notably after treated with both chemicals.

关 键 词：黄瓜 同工酶 性别分化 

分 类 号：S642.2[农业科学—蔬菜学]