采用RT-PCR扩增方法从犁苞滨藜中扩增NHX基因  

Using RT-PCR to Amplify the NHX Gene Fragment in Atriplex dimorphostegia

作　　者：李金耀[1] 张富春[1] 马纪[1] 蔡伦[1] 鲍勇刚[1] 王宾[1] 

机构地区：[1]新疆大学生命科学与技术学院分子生物学重点实验室

出　　处：《植物生理学通讯》2003年第6期585-588,共4页Plant Physiology Communications

基　　金：国家科技攻关西部科技行动项目 (No .2 0 0 1BA90 1A3 2 )

摘　　要：采用RT PCR扩增的方法 ,从新疆野生植物犁苞滨藜中克隆获得 1.7kb的cDNA片段 ,测序和序列分析表明该基因片段包含NHX基因完整的读码框架。序列同源性分析结果显示犁苞滨藜NHX基因与滨藜NHX基因同源性高达 95 % ,与碱蓬同源性达到 86 % ,与柑桔同源性为 88% ,与拟南芥同源性为 84 % ,表明它是植物中高度保守的一种基因 。The NHX gene encodes functional protein that may play an important role in plant salt tolerance. To clone the NHX from the wild plant Atriplex dimorphostegia in Xinjiang into a T-vector, designed primers were used to amplify 1.7 kb NHX cDNA fragment by RT-PCR. Sequence analysis revealed that the cloned NHX fragment of Atriplex dimorphostegia contained entire NHX coding region with 95%,86%,88% and 84% identity comparing with Atriplex gemelini, Suaeda maritima, Citrus×paradisi and Arabidopsis thaliana, respectively. This analysis suggested that NHX gene was highly conserved in terms of evolution in plant; and it also suggested that the NHX gene of Atriplex dimorphostegia was similar to that of Arabidopsis. It may be of great importance in improvement of the plant salt tolerance and breeding of crop.

关 键 词：犁苞滨藜 NHX基因 RT-PCR 植物耐盐 

分 类 号：Q943[生物学—植物学]