水稻OsTB1基因的结构及其表达分析(英文)  

The Analysis of the Structure and Expression of OsTB1 Gene in Rice

作　　者：胡文军[1] 张淑红[2] 赵忠[2] 孙崇荣[2] 赵毓橘[3] 罗达[1] 

机构地区：[1]中国科学院上海生命科学研究院植物生理生态研究所,植物分子遗传国家重点实验室,上海200032 [2]复旦大学生命科学学院生物化学系,上海200433 [3]中国科学院上海生命科学研究院植物生理生态研究所,上海200032

出　　处：《植物生理与分子生物学学报》2003年第6期507-514,共8页Journal Of Plant Physiology and Molecular Biology

基　　金：国家自然科学基金项目 (No .3 9870 3 99)资助~~

摘　　要：TCP基因是一类植物中新发现的、可能具有转录因子活性的基因家族 ,成员包括金鱼草的Cy clodiea (Cyc)、玉米的TeosinteBranched1 (TB1 )以及水稻中的PCF1、PCF2等。玉米的TB1基因有维持玉米顶端优势的作用 ,与分蘖的发生密切相关 ;水稻和玉米同属禾本科 ,在发育的过程中都有分蘖的发生。通过筛选水稻的基因组文库 ,得到了水稻中的一个TB1同源基因OryzasativaTeosinteBranched1 (OsTB1 )。该基因不含内含子 ,基因编码一个长度为 388个氨基酸的蛋白 ,在氨基酸水平上与TB1的同源性为 70 % ,含有保守的TCP区和R区 ,是属于TCP基因家族的一个成员。RT PCR和mRNA原位杂交分析结果表明 ,OsTB1在水稻的侧芽中有很强的表达 ,在花序中有较弱的表达。以上结果显示该基因可能在水稻侧芽和花序的起始和发育过程中起重要作用。TCP genes, including Teosinte Branched 1(TB1) in maize, Cyclodiea (Cyc) in snapdragon and PCF1 and PCF2 in rice etc., constitute a newly discovered gene family which encode transcription regulators in plants. TB1 controls the apical dominance in maize and is involved in the maize tiller development. Both rice and maize belong to the Gramineae and can produce tillers. By screening a rice genomic library, we isolated a TB1-like gene—Oryza sativa Teosinte Branched (OsTB1) in rice. We also isolated the cDNA of OsTB1 by RT-PCR. The OsTB1 gene, with no intron, encodes a polypeptide consisting of 388 amino acids. OsTB1 shows 70% amino acid sequence similarity with TB1 and has the conserved TCP and R regions. This gene is a newly discovered gene of the TCP gene family in rice. RT-PCR and mRNA in situ hybridization showed that OsTB1 strongly expressed in rice axillary buds and weakly in inflorescence. OsTB1 is suggested to be related to axillary bud and inflorescence development in rice.

关 键 词：水稻 发育 侧芽 OsTB1 顶端优势 

分 类 号：Q754[生物学—分子生物学]