微血管密度和p16基因表达对判定肾癌生物学行为的意义  

Correlation between intratumor microvessel density and p16 gene expression in renal cell carcinoma and their clinical implication

作　　者：贾瑞鹏[1] 马庆铮[2] 程继义[2] 许纯孝[2] 刘军[1] 王万忠[3] 杜德利[3] 王家耀[3] 

机构地区：[1]南京医科大学附属南京第一医院泌尿外科,南京210006 [2]山东省立医院泌尿外科 [3]山东省立医院病理科

出　　处：《临床泌尿外科杂志》2003年第11期650-652,共3页Journal of Clinical Urology

摘　　要：目的 :探讨肿瘤内的微血管密度 (IMD)和 p1 6基因表达与肾细胞癌 (RCC)生物学行为的关系。 方法 :采用免疫组织化学方法 ,对 76例RCC患者的根治性肾切除标本 ,检测第Ⅷ因子相关抗原和 p1 6基因表达 ,分析IMD、p1 6基因表达与RCC分期、分级及预后的相关性。 结果 :IMD随临床分期升高而增加 (P <0 .0 5 ) ,而与分级无明显相关性 (P >0 .0 5 ) ,随访 5年内死亡者IMD明显升高 (P <0 .0 1 ) ;癌旁组织中 p1 6阳性率 (75 .0 % )显著高于RCC组织 (4 8.7% ) ,p1 6阳性表达随临床分期、病理分级升高而降低 (P <0 .0 5 ) ,而与IMD呈负相关性。结论 :IMD是预测RCC恶性行为的一个有用指标 ,IMD和p1 6基因表达可为RCC的疗效和预后判定提供重要的资料。Purpose:To study the correlation among intratumor microvessel density (IMD), p16 gene expression, biological behavior,prognosis of renal cell carcinoma(RCC).Methods:Expression of IMD and p16 were detected in 76 cases of RCC tissues and 20 adjacent non neoplastic kidney tissues by immunohistochemical technique and the correlation among IMD, p16 gene expression, various clinicopathologic factors,and prognosis was studied.Results:IMD increased with Robson stage (P< 0.05 ) but had no relationship with Fuhrman grade (P> 0.05 ). IMD decreased greatly in the RCC patients whose survival was less than 5 years (P< 0.01 ). The positive rate of p16 was higher in non neoplasm tissues (75%) than that in tumor tissues ( 48.7 %),and the worse the stages and grades of RCC, the lower the p16 gene expression (P< 0.05 ). The p16 gene positive expression had negative correlation with IMD.Conclusions:IMD within RCC was an useful indicator for tumor behavior. IMD and p16 gene expression might identify RCC patients at higher risk for recurrence and poor prognosis.

关 键 词：肾肿瘤 微血管密度 P16基因 

分 类 号：R692[医药卫生—泌尿科学]