钾通道阻滞剂对大鼠支气管平滑肌细胞增殖的影响  

Effects of potassium channel blockers on the proliferation of rat bronchial smooth muscle cells

作　　者：刘先胜[1] 徐永健[1] 张珍祥[1] 倪望[1] 

机构地区：[1]华中科技大学同济医学院附属同济医院,湖北武汉430030

出　　处：《药学学报》2003年第5期333-336,共4页Acta Pharmaceutica Sinica

基　　金：国家自然科学基金资助项目( 30 2 70 583);教育部"高等院校骨干教师资助计划"2 0 0 0年度资助项目

摘　　要：目的　探讨电压依赖性延迟整流钾通道 (KV)、Ca2 +激活钾通道 (KCa)和ATP敏感性钾通道 (KATP)对大鼠支气管平滑肌细胞 (BSMC)增殖的影响。方法　应用免疫细胞化学、MTT微量比色分析法及流式细胞术 ,观察KV ,KCa和KATP对培养中大鼠BSMC增殖的影响。结果　KV 阻断剂 4 氨基吡啶 (4 AP)显著促进大鼠BSMC增殖细胞核抗原的表达 ,提高BSMC吸光度值 ,使S +G2 M期细胞数显著增多 ,并显著提高基础状态下BSMC内Ca2 +浓度 ,而KCa阻断剂四乙铵 (TEA)和KATP阻断剂格列本脲 (Glib)均无此效应。结论　大鼠BSMCKV 活性的抑制 ,可提高细胞内Ca2 +浓度 ,促进细胞的增殖 。Aim To investigate the effects of blockers of the three kinds of potassium channels: voltage dependent K + channel(K V), calcium activated K + channel(K Ca ) and ATP sensitive K + channel(K ATP ), on the proliferation of rat bronchial smooth muscle cells (BSMCs). Methods The effects of three kinds of potassium channel blockers on the proliferation of BSMCs were detected by MTT method, immunocytochemistry staining and flow cytometry. Their effects on the dynamic changes of Ca 2+ concen...

关 键 词：气道平滑肌细胞 钾通道 4-氨基吡啶 四乙铵 格列苯脲 

分 类 号：R965[医药卫生—药理学]