HCV/HBV复合DNA免疫的初步研究  

Preliminary study on the immunization effect of the combined HCV/HBV DNA immunization

作　　者：马绍辉[1] 刘龙丁[1] 董少忠[1] 王丽春[1] 陈丽珊[2] 任大明[2] 李琦涵[1] 

机构地区：[1]中国医学科学院中国协和医科大学医学生物学研究所,云南昆明650118 [2]上海复旦大学遗传学研究所,上海200433

出　　处：《免疫学杂志》2003年第S1期14-17,共4页Immunological Journal

摘　　要：目的 探讨HCV/HBV复合抗原PCX/S免疫原性。方法 将已证实具有良好免疫原性的人工合成的HCV复合多表位抗原基因PCX与HBV的S抗原基因融合于真核表达载体pcDNA3.0,构建真核表达载体pcDNA-PCXS。大量提取质粒并免疫小鼠,用ELISA的方法检测抗-HBs和抗-HCVAb;~3H-TdR渗入法检测免疫小鼠T淋巴细胞增殖;用^(51)Cr释放法检测免疫小鼠特异性CTLs杀伤作用。结果 免疫后均能检测到抗-HBsAb和抗-HCVAb,抗体水平随着时间的推移逐渐升高,但后者OD_(450nm)平均值低于抗-HBsAb的OD_(450nm)。免疫小鼠诱发针对PCX或S抗原的CTL反应和淋巴细胞转化。结论 复合型PCX/S抗原基因可诱发特异性免疫应答,为HCV/HBV双价疫苗的研究提供一定实验基础。Objective To explore the possibility of the hepatitis C virus / hepatitis B virus bivalent DNA immunization.Methods A synthetic multiple epitope antigen gene PCX of HCV fused with HBsAg gene was cloned into vector pcDNA3.0 to construct eukaryotic expression vectors pcDNA-PCXS. The target genes were sequenced and the plasmids were extracted, then they were used to immunize mice. The anti-HBs and anti-HCV antibodies were detected by ELISA, T lymphocytes proliferation response and the cytotoxicity of splenocyt...

关 键 词：丙型肝炎病毒 乙型肝炎病毒 DNA免疫 免疫应答 

分 类 号：R392[医药卫生—免疫学]