大鲵野生亲代与人工繁育子二代的随机扩增多态DNA分析(英文)  

RAPD analysis on the wild parents and second filial generation of artificial breeding of Andrias davidianus

作　　者：林茂[1] 黄景[2] 李正[1] 杨先乐[1] 黄俊海[2] 郑卫东[2] 黄艳平[1] 薛晖[1] 

机构地区：[1]上海水产大学农业部水产种质资源与养殖生态重点开放实验室,上海200090 [2]广东珠海金鲵野生动物养殖有限公司,广东珠海519015

出　　处：《上海水产大学学报》2003年第S1期20-23,共4页Journal of Shanghai Fisheries University

摘　　要：用15个能稳定扩增的随机引物对大鲵亲代与子二代各7个个体进行RAPD分析,结果表明,大鲵人工繁育子二代与野生亲代群体间具有较高的遗传相似度,其平均值为0.9515,这说明子二代群体在基因型上并没有发生明显的变异。遗传相似性和遗传多样性分析的结果表明,大鲵亲代与子二代的平均遗传距离分别为0.0497和0.0166,Shannon多样性指数分别为0.237和0.078,经t检验差异均极显著(P<0.01),这说明大鲵子二代遗传多样性水平低于亲代,因此必须加强谱系建立与繁育管理。The genetic distance, similarity and diversity of the parent and the second filial populations of Chinese giant salamanders Andrias davidianus were surveyed by RAPD analysis. The average genetic similarity between the parent and the second filial population was very high, which reached 0.9515. It showed that the second filial population hardly varied compared to the parent population. Relative genetic distances of the parent and the second filial population were 0.0497 and 0.0166, respectively; and the Shan...

关 键 词：大鲵 随机扩增多态DNA 遗传距离 遗传相似性 遗传多样性 

分 类 号：Q781[生物学—分子生物学]