抗前列腺特异性抗原单克隆抗体的制备、鉴定及初步应用  

Preparation, characterization and application of monoclonal antibodies for prostate-specific antigen

作　　者：张瑜[1] 吕琦[1] 王泽华[1] 袁航[1] 赵翠兰[1] 贾黎[1] 马以娟 王永祺[1] 张远旭[1] 马岚[1] 

机构地区：[1]云南大学单克隆抗体工程技术中心,云南昆明650091

出　　处：《云南大学学报（自然科学版）》2003年第S1期176-179,共4页Journal of Yunnan University(Natural Sciences Edition)

基　　金：云南省科委省院省校科技合作项目(99YB02).

摘　　要：通过杂交瘤技术制备抗前列腺特异性抗原(PSA)单抗细胞株,共获得68株稳定分泌PSA单克隆抗体的细胞株,并对其中与总PSA(t-PSA)反应的12株进行了详细的免疫特性鉴定.68 mouse hybridoma cell lines which stably secreted monoclonal antibodies against human prostate-specific antigen (PSA) were obtained through hybridoma technique. 12 among these McAbs which reacted with total PSA (tPSA) were identified for their immunological characters in detail. 

关 键 词：前列腺特异性抗原 亲和常数 单克隆抗体 抗原表位 

分 类 号：Q813.2[生物学—生物工程]