乌三颗粒体外对恶性肿瘤细胞核酸合成的抑制作用  

Effect of Wusan Granule in Inhibiting the Nucleic Acid Synthesis of Tumor Cell in vitro

作　　者：石锦萍[1] 钟柏松[2] 高丽元[1] 臧志和[1] 辛志伟[1] 沈映君[2] 黄秀风[2] 

机构地区：[1]第三军医大学成都军医学院人体结构教研室,成都610083 [2]成都中医药大学

出　　处：《中国中西医结合杂志》2003年第S1期142-144,共3页Chinese Journal of Integrated Traditional and Western Medicine

基　　金：重庆科委立项课题(No.1999);四川省教委青年基金重点课题(No.2000-A71)

摘　　要：目的:探讨中药复方制剂乌三颗粒在体外对肿瘤细胞增殖的影响。方法:将人巨细胞肺癌、人肝细胞癌、人肺腺癌细胞株在体外培养传代3次后,各组分别加入受试药物,~3H-TdR 掺入法检测肿瘤细胞核酸的合成,琼脂集落法检测肿瘤干细胞增殖的能力。结果:乌三颗粒剂量在0.5～8mg/ml 时,可明显减少人巨细胞肺癌、人肝细胞癌、人肺腺癌细胞三株细胞对~3H 标记的胸腺嘧啶核苷(~3H-TdR)的摄入,明显减少肿瘤干细胞集落的形成,与对照组比较差异有显著性(P<0.01)。结论:乌三颗粒在体外对多株肿瘤细胞的核酸合成有显著抑制作用,同时有抑制肿瘤干细胞的增殖能力。Objective:To study the in vitro effect of Wusan Granule (WSG) on tumor cell proliferation.Methods:After cell lines of human giant cell carcinoma of lung,human liver carcinoma and human adenocarcinoma of lung were cultured in vitro for three generations, they were treated in groups,and the nucleic acid synthesis in tumor cells were measured by ~3H-TdR coorporation and the prolifera- tion of tumor stem cell was detected by agar colony method.Results:WSG with dosage within 0.5-8 mg/ml could significantly de- c...

关 键 词：乌三颗粒 瘤细胞株 细胞培养 核酸合成 细胞集落 

分 类 号：R285.5[医药卫生—中药学]