葛根素对大鼠脑缺血再灌注细胞间粘附分子-1表达的影响  

Effect of Puerarin on Expression of Intercellular Adhesion Molecule-1 in Cerebral Ischemia/Reperfusion Rats

作　　者：杨黎[1] 何世银 

机构地区：[1]华中科技大学同济医学院附属协和医院,武汉430022

出　　处：《中国中西医结合杂志》2003年第S1期42-44,共3页Chinese Journal of Integrated Traditional and Western Medicine

摘　　要：目的:探讨葛根素抗缺血再灌注损伤的机制是否与其抑制细胞间粘附分子-1(ICAM-1)表达相关。方法:采用线栓法制备大鼠局灶性大脑中动脉栓塞(MCAO)模型,以免疫组化的方法观察葛根素对大鼠脑缺血再灌注后血管内皮细胞 ICAM-1蛋白表达的影响,并以比色法观察其对脑组织中髓过氧化物酶(MPO)活性的影响。结果:葛根素能有效地抑制再灌注各时间点的 ICAM-1蛋白的过量表达,并抑制 MPO的活性(P<0.05或 P<0.01)。结论:葛根素可下调缺血再灌注后脑组织中 ICAM-1蛋白的表达,并可抑制MPO 的活性。Objective:To investigate whether the anti-ischemia/reperfusion effect of puerarin is related to its ifihibition on the expression of intercellular adhesion molecule-1 (ICAM-1).Methods:The focal cerebral ischemia reperfusion model was established by means of thread ligation caused middle cerebral artery occlusion (MCAO) and embolism,ICAM-1 was determined and localized by immunohis- tochemistry method and the effect on activity of myeloperoxidase enzyme(MPO) in brain tissue was measured with colorimetry metho...

关 键 词：葛根素 缺血再灌注损伤 细胞间粘附分子-1 髓过氧化物酶 

分 类 号：R285.5[医药卫生—中药学]