麝香酮对急性脑缺血大鼠海马神经元谷氨酸转运体EAAC_1基因表达的影响  

Effect of Muscone on Gene Expression of Neuronal Glutamate Transporter EAAC_1 of Acute Cerebral Ischemic Hippocampus in Rats

作　　者：梁辉[1] 蔡定芳[1] 陈虎[1] 刘静[1] 沈思钰[1] 赵刚[1] 

机构地区：[1]复旦大学附属华山医院中西医结合研究所,上海200040

出　　处：《中国中西医结合杂志》2003年第S1期40-42,共3页Chinese Journal of Integrated Traditional and Western Medicine

摘　　要：目的:研究麝香酮对大鼠局灶性脑缺血模型不同缺血时间海马神经元谷氨酸转运体 EAAC_1在转录水平表达的影响。方法:用插线法建立大鼠局灶性脑缺血模型前0.5h 予灌服麝香酮,剥取海马,采用半定量逆转录-聚合酶链式反应(RT-PCR),测定 EAAC_1 mRNA 水平的变化。结果:海马 EAAC_1 mRNA 在缺血后6、24h 明显增加,缺血1h 没变化,麝香酮可有效下调其表达。结论:麝香酮可有效下调 EAAC_1mRNA 在缺血海马的表达。Objective:To investigate the effect of muscone on neuronal glutamate transporter EAAC_1 transcriptional level at different is- chemic times in acute ischemic hippocampus in rats.Methods:Focal ischemic models of middle cerebral artery occlusion were achieved by inserting nylon surgical thread through the internal carotid artery into the anterior cerebral artery.Brain samples were harvested from ischemic hippocampus.The expression of EAAC_1 was assessed by RT-PCR.Results:EAAC_1 mRNA were not changed in the hi...

关 键 词：麝香酮 脑缺血 海马 神经元谷氨酸转运体 转录 

分 类 号：R285.5[医药卫生—中药学]