高脂饲料结合免疫损伤、应激所致高脂血症血瘀模型的实验研究  

Experimental Study on Hyperlipidemia and Blood Stasis Model Induced by High Cholesterol Diet Plus by Immunologic Injury and Stress

作　　者：刘剑刚[1] 张红霞[1] 董国菊[1] 杨晓红[1] 许勇钢[1] 史大卓[1] 

机构地区：[1]中国中医研究院西苑医院,北京100091

出　　处：《中国中西医结合杂志》2003年第S1期17-21,共5页Chinese Journal of Integrated Traditional and Western Medicine

基　　金：国家重点基础研究发展规划项目课题(No.G1999054405)

摘　　要：目的:采用高脂、免疫损伤、应激等方法,探讨较为切合动脉粥样硬化(AS)病理变化的高脂血症血瘀动物模型造模方法。方法:应用单纯高胆固醇(模型组简称高脂组)、异体血清蛋白加高胆固醇(免疫损伤高脂组)、高胆固醇加异体血清蛋白加肾上腺素(免疫损伤应激高脂组)、单纯肾上腺素(应激组)等不同方法造模,观察不同模型脂质代谢、血小板功能、白细胞活化粘附、白细胞介质、血管内皮功能的变化。结果:与空白组比较:高脂组大鼠的脂质代谢、血管活性物质、炎症介质明显升高(P<0.05,P<0.01);应激组大鼠的白细胞活化和血小板活化显著升高(P<0.05,P<0.01);免疫损伤高脂组血管活性、脂质代谢、炎症介质、白细胞活化显著增高(P<0.05,P<0.01);免疫损伤应激高脂组的血小板活化显著升高,血小板聚集性增强,白细胞活化增强,炎症介质、脂质代谢变化异常,增高显著(P<0.05,P<0.01)。结论:多因素的高脂血症血瘀模型的脂质代谢、白细胞介素、白细胞粘附与活化及血小板活化、聚集、粘附程度都显著升高,为中医药多环节干预 AS 和血瘀证的作用研究提供了一个较为可靠的模型。Objective:Methods of high fat forage,immunologic injury and stress,etc.were used in this experiment to explore a suitable method in establishing animal model of hyperlipidemia and blood stasis for the study of arteriosclerosis (AS).Methods:Models were established by different methods.Rats were fed by single high cholesterol diet (HC group) in group A,fed by high cholesterol diet and injected with xenoma serum protein (HCD-XSP)in group B,fed by high cholesterol diet and injected with xenoma serum protein and...

关 键 词：高脂血症 免疫损伤 应激 血瘀模型 脂质代谢 白细胞介素 血小板功能 血管活性物质 

分 类 号：R589.2[医药卫生—内分泌]