天舒胶囊对偏头痛大鼠血浆β内啡肽、五羟色胺含量及其脑组织c-fos表达的影响  

Effects of Tianshu capsule on the plasma levels of β-endorphin and 5-Hydroxytryptamine and c-fos expression of brain tissue in rats with migraine

作　　者：杨天华[1] 张勤[1] 周沐科[1] 刘永宏[1] 周东[1] 

机构地区：[1]四川大学华西医院神经内科,成都610041

出　　处：《临床神经病学杂志》2008年第5期368-370,共3页Journal of Clinical Neurology

摘　　要：目的研究天舒胶囊对偏头痛大鼠血浆β内啡肽(β-EP)、五羟色胺(5-HT)含量及其脑组织c-fos表达的影响。方法皮下注射硝酸甘油建立偏头痛大鼠模型,给予天舒胶囊高、中、低剂量灌胃(剂量为7.5g/kg、3.75g/kg和1.88g/kg),按治疗和预防两种方式给药。空白对照组和模型组予以相应剂量的生理盐水灌胃。采用放免法和高效液相色谱法分别测定大鼠静脉血中β-EP、5-HT含量;免疫组化染色观察中脑导水管周围灰质β-EP、5-HT和c-fos阳性表达。结果与模型组比较,天舒胶囊高剂量治疗和预防组、中剂量预防组血浆β-EP水平明显升高(均P<0.05),天舒胶囊高、中、低剂量治疗和预防组血浆5-HT水平明显降低(均P<0.05);天舒胶囊中剂量治疗和预防组中脑导水管周围灰质c-fos表达明显降低(均P<0.05),而β-EP、5-HT表达增高(均P<0.05)。结论天舒胶囊可改善偏头痛发作时血管活性物质和神经递质水平失常,从而改善偏头痛症状。Objective To investigate the effects of Tianshu capsule(TSC) on the plasma levels of β-endorphin(β-EP) and 5-Hydroxytryptamine(5-HT) and c-fos expression of brain tissue in the rat with migraine.Methods The rat migraine model was established by subcutaneously administration of nitroglycerin.The intragastric administration dose of TSC was divided into three groups as follows:high dose(7.5 g/kg),middle dose(3.75 g/kg) and low dose(1.88 g/kg).TSC was administrated respectively in therapeutic and prophylactic m...

关 键 词：天舒胶囊 β-内啡肽 5羟色胺 c-fos 偏头痛 

分 类 号：R285.5[医药卫生—中药学]