胡枝子属植物ITS序列研究与系统发育分析  

Phylogenetic Relationship of Genus Lespedeza by ITS Sequence Data

作　　者：王秀荣[1] 赵杨[1] 骈瑞琪[2] 陈晓阳[2] 

机构地区：[1]贵州大学林学院,贵州贵阳550025 [2]北京林业大学,北京100083

出　　处：《西北林学院学报》2008年第5期70-73,共4页Journal of Northwest Forestry University

基　　金：国家高技术研究发展计划(863计划)(2002AA241111);贵州省优秀科技教育人才省长专项基金(Z073224)

摘　　要：用PCR产物直接测序法对10种胡枝子属植物和1个外类群的ITS序列进行了测定。结果表明,10种胡枝子ITS序列全长在612～622bp之间,其中ITS1长度在243～228bp之间,变异较大,ITS2长度在215～220bp,5.8S的长度均为165bp,比较保守。序列比对结果发现有76个变异位点,占比对序列长度的11.93%。构建了胡枝子属植物ITS序列的系统发育树,并探讨了参试胡枝子种质的亲缘关系。ITS sequences of ten kinds of 10s plants of Lespedeza and an outgroup were obtained by primer design PCR,sequencing and cluster analysis.The results showed that ITS1 section length was 228~243 bp 5.8S sequence length was 165 bp which was too conservative,and the ITS2 section length varied from 215 bp to 220 bp and the conservative sites occupied 88.1%.Phylogenetic analysis of ITS sequences using PAUP 4.0 software and their genetic relationship was discussed

关 键 词：胡枝子属 ITS序列 系统发育 

分 类 号：S541.5[农业科学—作物学]