耐亚胺培南铜绿假单胞菌耐药机制研究  

Mechanism of imipenem resistance in Pseudomonas aeruginosa

作　　者：应春妹[1] 应骏[1] 罗柳林[1] 汪雅萍[1] 叶杨芹[1] 张灏旻[1] 

机构地区：[1]上海交通大学医学院附属仁济医院检验科,上海浦东200127

出　　处：《中国感染与化疗杂志》2008年第4期300-302,共3页Chinese Journal of Infection and Chemotherapy

基　　金：上海浦东新区科技发展基金创新资金创新攻关项目(PKJ2006-Y07)

摘　　要：目的了解我院分离的耐亚胺培南铜绿假单胞菌(PA)耐药机制及同源性分型情况。方法收集临床分离耐亚胺培南PA,用K-B法检测28株耐亚胺培南PA对12种抗菌药的敏感性,用肠杆菌科细菌基因间重复序列(ERIC)进行同源性分析,并用PCR法检测外膜孔蛋白OprD2和金属β内酰胺酶(IMP、VIM)基因。结果28株耐亚胺培南PA对其他12种抗菌药的耐药率在14.8%~42.3%。经ERIC-PCR分析后共有11个基因型。其中A型为11株:A1型9株,A2型2株;B型为5株:B1型2株,B2型2株,B3型1株;C型3株;D型2株;E^K型各1株。PCR检测到OprD2基因仅4株,其缺失率为85.7%,28株PA中检测到1株VIM基因,经测序证实为VIM-2型。结论本院耐亚胺培南PA的主要耐药机制为外膜孔蛋白缺失。在外科和高干病房中出现多种基因型的耐亚胺培南PA传播。Objective To study the mechanism of imipenem resistance in Pseudomonas aeruginosa,and investigate its molecular epidemiology by ERIC-PCR.Methods Twenty-eight strains of imipenem-resistant P.aeruginosa were isolated from patients in Renji Hospital.The antimicrobial susceptibility was determined by K-B method.The homology among those isolates was analyzed by enterobacterial repetitive intergenic consensus(ERIC).The genes of metallo enzyme IMP,VIM,as well as OprD2 encoding outer membrane porin,were analyzed wi...

关 键 词：耐亚胺培南 铜绿假单胞菌 外膜孔蛋白 金属Β内酰胺酶 肠杆菌科细菌基因间重复序列 

分 类 号：R516[医药卫生—内科学]