沙棘叶片不定芽再生的研究  

Studies on the Adventitious Shoot Regeneration from the Leaves of Hippophae rhamnoides L.

作　　者：张端伟[1] 宋西德[1] 张宗勤[1] 张永[1] 周锋利[1] 潘静[1] 

机构地区：[1]西北农林科技大学林学院,陕西杨陵712100

出　　处：《西北林学院学报》2008年第4期93-96,共4页Journal of Northwest Forestry University

基　　金：陕西省农业攻关项目(2007K01-11-01)

摘　　要：以"实优1号"沙棘枝条水培芽叶片为试材,研究了叶片着生节位、同一叶片不同部位、不同浓度比值的6-BA与IAA对愈伤组织产生和不定芽再生的影响。结果表明,适宜水培叶片愈伤组织产生的外植体材料为水培茎尖上部与中部叶片的基部和中部,适宜诱导不定芽的外植体为下部叶片与中部叶片;同一叶片不同部位对不定芽再生率影响研究表明叶片中部为首选材料;可迅速高效的诱导不定芽发生的培养基为1/2MS+6-BA 0.7 mg/L+IAA0.5 mg/L,平均每外植体分化不定芽数达到6.75个。Using hydroponics stem apexes of cultivar "Shiyou 1" of Hippophae rhamnoides as explants,different locations on the stem apexes,different parts of the of leaves and different concentrations of 6-BA with IAA were tested for their optimal effects on inducing callus and adventitious shoot regeneration.The results showed that the middle and proximal parts of the leaves from the upper and middle parts of the stem apexes were easier to induce callus,the upper and the middle parts of leaves were easier to induce a...

关 键 词：沙棘 叶片 不定芽 再生 

分 类 号：S793.6[农业科学—林木遗传育种]