过路黄的组织培养及叶片植株再生  

Tissue Culture and Plant Regeneration from the Leaves of Lysimachia christinae

作　　者：王艳[1] 杜鸿云[2] 梁海永[3] 刘桂林[1] 

机构地区：[1]河北农业大学园林与旅游学院,河北保定071001 [2]河北农业大学,河北保定071001 [3]河北农业大学林学院,河北保定071001

出　　处：《西北林学院学报》2008年第4期104-108,共5页Journal of Northwest Forestry University

基　　金：河北省交通厅重点攻关项目(Y-050116)

摘　　要：用过路黄嫩茎作为外植体进行组织培养,在附加不同浓度激素的培养基上对其进行增殖、生根培养,诱导叶片愈伤组织的形成及叶片愈伤组织不定芽的形成,建立其叶片再生体系。结果表明,过路黄最适分化培养基是MS+6-BA 0.5 mg/L+NAA 1.0 mg/L,最适生根培养基是不附加任何激素的1/2MS培养基,诱导叶片愈伤组织最适培养基是MS+6-BA 0.3 mg/L+NAA 0.1mg/L,诱导叶片愈伤组织不定芽形成最适培养基是MS+6-BA 0.3 mg/L+NAA 0.1 mg/L。The infancy stem segments of Lysimachia christinae were taken as explants to be cultured in the media supplemented with different levels of hormone to investigate its proliferation,rooting,formation of leaf callus and adventitious bud from leaf callus,and to establish leaf regeneration system.The resulted showed that preferable propagation medium was MS+6-BA 0.5 mg/L+NAA 1.0 mg/L,rooting medium was 1/2MS,medium for leaf callus induction was MS+6-BA 0.3 mg/L+NAA 0.1 mg/L.

关 键 词：过路黄 组织培养 叶片再生 

分 类 号：S68[农业科学—观赏园艺]