F_1代法监测田间棉铃虫对转Bt基因棉的抗性  

Monitoring resistance to transgenic Bt cotton in field populations of Helicoverpa armigera (Hübner) (Lepidoptera:Noctuidae) with F1 screening method

作　　者：刘凤沂[1] 朱玉成[2] 沈晋良[1] 

机构地区：[1]南京农业大学植物保护学院农药科学系农业部病虫监测与治理重点开放实验室 [2]Jamie Whitten Delta States Research Center,USDA-ARS,Stoneville

出　　处：《昆虫学报》2008年第9期938-945,共8页Acta Entomologica Sinica

基　　金：国家自然科学基金项目(30270889)

摘　　要：采用表达单价Cry1Ac毒素的转Bt基因棉棉叶喂饲法比较了棉铃虫Helicoverpa armigera(Hbner)抗、感亲本及正反交后代在Bt棉上5d的发育速率,确定棉铃虫在Bt棉上5d发育到2龄中期以上、体重≥0.6mg的个体作为判别抗性纯合子的标准。2006年采用F1代法在室内用Bt棉叶喂饲法检测河北省邱县Bt棉田棉铃虫对Bt棉的抗性等位基因频率。结果表明,127头田间雄虫中24头携带抗性基因,估测抗性等位基因频率为0.94(95%CI:0.044～0.145),该值为在国内首次检测到的高抗性等位基因频率。该地区长期大面积种植转单价Bt棉和缺少非Bt棉作为有效庇护区导致田间抗性快速进化,需要尽快采取有效的抗性治理策略。本文还讨论了影响大田产生抗性的因素以及田间存在的抗性风险等。Development of Bt-susceptible and resistant Helicoverpa armigera and their reciprocal crosses feeding on Bt cotton leaves for 5 d were compared,and individuals ≥0.6 mg/larva and surviving beyond the mid-2nd instar on Bt cotton leaves for 5 d were considered as the standard for homozygous resistant individuals.In 2006,the frequency of allele conferring resistance to Bt cotton in Qiuxian County,Hebei Province,was estimated by using the F_1 screening method.The results showed that 24 out of 127 field-collected...

关 键 词：转BT基因棉 棉铃虫 抗性监测 F1代法 

分 类 号：S435.621.2[农业科学—农业昆虫与害虫防治]