BDNF、TrkB在大鼠海马CA_3区的表达及其与学习记忆的关系  

The expression of BDNF and TrkB in rat CA_3 and their relationship with leaning and memory

作　　者：宁晓霞[1] 师社会[2] 邢亮[2] 刘涛[2] 张海英[2] 胡海涛[2] 

机构地区：[1]西安医学院附属医院神经内科,西安710077 [2]西安交通大学医学院附设卫校

出　　处：《陕西医学杂志》2008年第9期1130-1132,共3页Shaanxi Medical Journal

基　　金：陕西省卫生厅科研基金项目(No06D-12)

摘　　要：目的:研究BDNF及其受体TrkB在大鼠海马CA3区时空分布变化及其与学习记忆的关系。方法:采用免疫组织化学实验,观察BDNF及TrkB在正常大鼠发育期、成年期和老龄期海马CA3区的定位分布及时间变化。成年组和老龄组于免疫组织化学染色前接受水迷宫试验,研究BDNF及TrkB的分布变化与学习记忆的关系。结果:生后15~20d,海马CA3区阳性细胞最多。成年期细胞染色强,核大而圆无着色。老龄期阳性细胞数量少,染色淡,发生了明显退行性变化。水迷宫定向航行实验中平均潜伏期成年组和老龄组有显著性差异(P<0.01);空间搜索实验中NE象限停留时间成年组和老龄组有显著性差异(P<0.01)。结论:海马CA3区BDNF及TrkB的时空表达基本一致。表达高峰为生后15~20d。老龄期呈增龄性下降,细胞出现退行性变化。BDNF及受体TrkB的表达与学习记忆能力呈平行关系,BDNF及受体TrkB在学习记忆中发挥着重要作用。Objective:To observe systematically the distribution of BDNF and TrkB in the normal rat CA3 during development phase and adulthood and aged phase .To study the patterns of BDNF and TrkB and their relationship with leaning and memory. Methods:The expression of BDNF and TrkB in the normal rat CA3 during development phase and adulthood phase and aged phase and thire relationship with leaning and memory were studied by methods of immunohistochemistry and Morris water maze test. Results :BDNF and TrkB immunoreac...

关 键 词：海马/病理生理学 神经生长因子类/代谢 受体 TRKB 学习记忆 免疫组化 动物 实验 大鼠 

分 类 号：R33[医药卫生—人体生理学]