丙酸氟替卡松对哮喘小鼠气道壁胶原沉积及MMP-9表达的影响  

Effects of fluticasone propionate on the collagen deposition and MMP-9 expression in asthmatic murine

作　　者：黄妙毅[1] 王睿[1] 

机构地区：[1]西安市中心医院呼吸内科,西安710003

出　　处：《陕西医学杂志》2008年第9期1133-1135,1203,共4页Shaanxi Medical Journal

摘　　要：目的:观察丙酸氟替卡松对哮喘小鼠模型气道壁胶原、基质金属蛋白-9(MMP-9)含量的影响。方法:将30只BALB/c小鼠随机分为哮喘组(A组)、正常对照组(B组)、丙酸氟替卡松治疗组(C组)3组,每组动物10只。制备小鼠肺组织病理切片,HE染色观察各组气道结构改变情况,天狼猩红染色,采用医学图像分析软件测定气道Ⅰ、Ⅲ型胶原的面积,肺组织石蜡切片行MMP-9免疫组化染色后计算机图像分析测定其灰度值。结果:光镜下可见A组小支气管上皮细胞脱落、管壁炎症细胞浸润、杯状细胞增生、基底膜增厚、平滑肌增厚,而C组上述改变较A组明显减轻。A组支气管壁Ⅰ型、Ⅲ型胶原较B组增多(P<0.05),而丙酸氟替卡松干预后C组较A组显著降低(P<0.05);A组MMP-9灰度值较B组明显降低(P<0.05),丙酸氟替卡松干预后的C组MMP-9灰度值较A组显著增高(P<0.05)。结论:吸入丙酸氟替卡松可以减少气道壁Ⅰ型、Ⅲ型胶原的沉积和MMP-9的表达,对胶原沉积的抑制可能是通过抑制MMP-9表达而实现。Objective:To investigate the influence of fluticasone propionate (FP) on the collagen deposition and expression of matrix metalloproteinases-9 in murine models of asthma.Methods: Thirty BALB/c mice were randomly divided into 3 groups: model group(group A), normal control group(group B),FP treatment group(group C), with 10 mice in each group. The airway inflammation and the alteration of airway structure were detected by HE staining. The distribution and the area of type Ⅰand type Ⅲ collagen in the lung tiss...

关 键 词：哮喘/病理学 气管疾病/病理学 哮喘/药物疗法 胶原/代谢 明胶酶B/代谢 糖皮质激素类/治疗应用 动物 实验 小鼠 

分 类 号：R562.25[医药卫生—呼吸系统]