REV感染肉种鸡后动态病理学观察与抗原分布及检测  

Dynamic observation on pathohistologic lesions and antigen location by PCR in broiler breeder infected with REV

作　　者：朱国[1] 刁秀国[1] 成子强[1] 王桂花[1] 孟祥凯[1] 高婷婷[1] 

机构地区：[1]山东农业大学动物科技学院,山东泰安271018

出　　处：《中国兽医学报》2008年第9期1065-1069,共5页Chinese Journal of Veterinary Science

基　　金：中华人民共和国博士后基金资助项目(2005038258)

摘　　要：用网状内皮组织增生病病毒(REV)人工接种1日龄肉种鸡,定期剖杀,通过病理组织学观察病变特征及肿瘤的发生发展;用免疫组化、PCR检测抗原与病毒在体内各器官的分布规律;超薄切片观察肿瘤细胞、病毒的形态。结果显示,肝脏、脾脏、胸腺、法氏囊较早出现明显的肿瘤增生灶,在这些器官内免疫组化阳性信号出现最早,且信号最强,在5周龄时,心肌、腺胃、肺、肾、骨髓等器官才开始出现阳性信号,但呈中度的阳性反应,9周龄后阳性信号开始减弱。肝脏、脾脏、胸腺、法氏囊、骨髓扩增出REV的LTR基因片段,超薄切片显示病毒多分布在胞浆内和细胞间隙内。研究发现病变明显的组织中,阳性反应较强,肿瘤细胞增生明显,即强阳性反应的组织器官易形成肿瘤转移灶及肿瘤结节,同时,抗原最早出现在这些器官说明其中可能存在REV的靶细胞。虽然阳性信号在腺胃出现较晚,但90%以上的腺胃肿大说明腺胃也是REV的主要侵害器官。One-day-old broiler breeders were inoculated with strain SNV of REV and slaughtered at different period.The process of lesion and tumor were study by pathohistologic detection.The distribution of antigen and virus in different organs were detected by immunohistochemistry and PCR.Tumor cell and morphous of virus were observed by electron microscope.The results showed that tumor nodule emerged earlier in liver,spleen,thymus and bursa than in other organs.And the most positive signal were found in these organs...

关 键 词：网状内皮组织增生病病毒 病理组织学 抗原分布 PCR 电镜观察 

分 类 号：S858.31[农业科学—临床兽医学]