貉α-卫星DNA的克隆及序列分析  

Cloning and sequence analysis of alpha satellite DNA in raccoon dog

作　　者：闫守庆[1] 范宗兴[1] 祝万菊[1] 邢沈阳[1] 赵志辉[1] 孙金海[1] 

机构地区：[1]吉林大学畜牧兽医学院,吉林长春130062

出　　处：《中国兽医学报》2008年第9期1105-1108,共4页Chinese Journal of Veterinary Science

基　　金：国家自然科学基金资助项目(30270948)

摘　　要：根据蓝狐、银狐及犬α-卫星DNA序列设计引物RSP1和RSP2,在貉基因组中经PCR扩增获得5条分子质量大小不同的DNA片段,分别命名为RSP1~RSP5,经克隆及测序获得14个RSP序列;序列分析表明,除RSP4外,同类RSP片段的不同序列的长度差异很小且同源性大于90%,不同种类的RSP片段的长度差异很大,最大为1 815 bp,最小为495 bp,但所有序列的3′端约500 bp序列都具有高度的同源性,大于90%;经Blast检索,所克隆的RSP序列的部分区域与蓝狐、银狐及犬α-卫星DNA序列具有约75%的同源性,证明所克隆的RSP序列为貉α-卫星DNA序列,但其单体大小及亚重复的组成还需进一步研究。A pair of primers,that is RSP1 and RSP2,were designed according to the alpha satellite monomers of Arctic fox,Silver fox and domestic dog.Five bands,designated as RSP1-RSP5,were achieved by PCR amplification in the genome of raccoon dog.PCR products were cloned and sequenced,and fourteen RSP sequences were obtained.The cloned sequences were variable in length,ranging from 495 to 1 815 bp,but the sequence had highly(90%) homology to each other among the same type of RSP fragment except the RSP4 sequences.App...

关 键 词：犬科动物 貉 卫星DNA 

分 类 号：S865.24[农业科学—野生动物驯养]