Improved speciation of dissolved organic nitrogen in natural waters: amide hydrolysis with fluorescence derivatization  

作　　者：Ryan L.Fimmen Tamara D.Trouts Daniel D.Richter Jr. Dharni Vasudevan 

机构地区：[1]School of Earth Sciences, The Ohio State University, 125 S.Oval Mall,Columbus, OH 43210, USA.Nicholas School of the Environment and Earth Sciences, Duke University, Durham, NC 27708, USA [2]School of Earth Sciences, The Ohio State University, 125 S.Oval Mall,Columbus, OH 43210, USA [3]Nicholas School of the Environment and Earth Sciences, Duke University,Durham, NC 27708, USA. [4]Nicholas School of the Environment and Earth Sciences, Duke University,Durham, NC 27708, USA.Department of Chemistry, Bowdoin College,Brunswick, ME 04011, USA

出　　处：《Journal of Environmental Sciences》2008年第10期1273-1280,共8页环境科学学报（英文版）

基　　金：the Andrew Mellon Foundation,NSF Biocomplexity Program (Richter);NSF Career Grant BES-9984489(Vasudevan);and the EPA-STAR program (Fimmen) for funding.

摘　　要：The objective of this study was to improve primary-amine nitrogen (1°-N) quantification in dissolved organic matter (DOM) originating from natural waters where inorganic forms of N, which may cause analytical interference, are commonly encountered. Efforts were targeted at elucidating organic-N structural criteria influencing the response of organic amines to known colorimetric and fluorescent reagents and exploring the use of divalent metal-assisted amide hydrolysis in combination with fluorescence analyse...The objective of this study was to improve primary-amine nitrogen (1°-N) quantification in dissolved organic matter (DOM) originating from natural waters where inorganic forms of N, which may cause analytical interference, are commonly encountered. Efforts were targeted at elucidating organic-N structural criteria influencing the response of organic amines to known colorimetric and fluorescent reagents and exploring the use of divalent metal-assisted amide hydrolysis in combination with fluorescence analyse...

关 键 词：dissolved organic matter/nitrogen （DOM/DON） O-PHTHALDIALDEHYDE FLUORESCAMINE NINHYDRIN amino acids amide hydrolysis peptides 

分 类 号：X132[环境科学与工程—环境科学]